3GPHSKB
Roche
KAPA3G Plant PCR Kit
Synonym(s):
PCR, Plant PCR
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All Photos(4)
About This Item
UNSPSC Code:
41106300
NACRES:
NA.55
usage
sufficient for 250 reactions
50 μL sufficient for 250 reactions (KK7251)
sufficient for 500 reactions
50 μL sufficient for 500 reactions (KK7252)
Quality Level
shelf life
≤12 mo.
feature
Difficult Templates/Specialty Enzymes PCR
dNTPs included: no
hotstart: no
manufacturer/tradename
Roche
technique(s)
PCR: suitable
input
crude DNA
storage temp.
−20°C
General description
KAPA3G Plant PCR Kits are designed for PCR of plant-derived DNA, using either purified DNA or DNA prepared by crude extraction methods (crude sample PCR). In addition, the KAPA3G Plant PCR Kit can be used to amplify DNA from plant material added directly to the PCR (direct PCR). The KAPA3G Plant PCR Kit contains KAPA3G DNA Polymerase, a novel, third-generation (3G) enzyme which was engineered via a process of directed evolution for improved tolerance to common plant-derived PCR inhibitors such as polyphenolics and polysaccharides.The KAPA3G Plant PCR Kits are optimized for fast and efficient amplification of plant DNA from crude samples, DNA-containing carry-over inhibitors from crude extraction methods, and purified DNA.
KAPA3G Plant DNA Polymerase is a blend of the engineered A-family KAPA3G DNA Polymerase and a modified B-family DNA Polymerase. The enzyme blend is combined with proprietary antibodies to inactivate the enzymes prior to the first denaturation step. The fidelity of the KAPA3G Plant DNA Polymerase is 4-10 times higher than that of wild-type Taq.
KAPA3G Plant DNA Polymerase is a blend of the engineered A-family KAPA3G DNA Polymerase and a modified B-family DNA Polymerase. The enzyme blend is combined with proprietary antibodies to inactivate the enzymes prior to the first denaturation step. The fidelity of the KAPA3G Plant DNA Polymerase is 4-10 times higher than that of wild-type Taq.
Application
KAPA3G Plant PCR Kit has been used for:
- Amplification of fragments up to 5 kb in size from purified plant DNA, extracted with commercial kits or cetyl trimethyl ammonium bromide (CTAB)-based methods
- Direct polymerase chain reaction (PCR) from leaf discs, seed samples, and other plant tissue types
- PCR from crude plant DNA extracts, prepared from leaf and/or seed material
- amplification of 16SrRNA
- an ultra-rapid real-time reverse transcriptase (RT)-PCR assay
Features and Benefits
Key features of the KAPA3G Plant PCR Kit include:
Amplify long targets from crude samples and purified DNA:
Perform direct PCR from a variety of plant species and tissue types:
Streamline workflows and improve turnaround times:
DNA extractions Improve success rates with novel crude sample plant PCR workflow:
Quick Notes:
- Fast PCR direct from plant tissues such as leaf discs, seeds and crude plant extracts.
- Streamlined workflows for transgenic screening.
- Improved PCR success rates and reproducibility.
- Efficient amplification of long and difficult targets from all sample types.
Amplify long targets from crude samples and purified DNA:
- Amplify fragments up to 5 kb from purified DNA and crude samples
- High yield and specificity with purified DNA and crude samples
Perform direct PCR from a variety of plant species and tissue types:
- Direct PCR with leaf disc or seed as template
- No need for time-consuming DNA extractions
Streamline workflows and improve turnaround times:
- Perform PCR in half the time compared with wild-type enzymes
- Eliminate the need for time-consuming
DNA extractions Improve success rates with novel crude sample plant PCR workflow:
- Use extraction buffer to prepare crude extracts for plant PCR in just 5 minutes
- High success rates with even the most challenging sample types
Quick Notes:
- KAPA3G Plant DNA Polymerase is tolerant to plant-derived PCR inhibitors, and can amplify frompurified DNA, crude extracts, and plant material.
- Optimize reaction conditions using purified DNA before attempting direct or crude sample PCR.
- For direct PCR, use a sampling tool to control the amount of plant material added to the reaction. The use of excessive amounts of crude plant material in PCR is a major cause of direct PCR reaction failure.
- For crude sample PCR, prepare a crude DNA extract using a small amount of plant material in Extraction Buffer (Refer to Section 3: Crude sample PCR), and use 1 μL per 50 μL reaction.
- KAPA Plant PCR Buffer contains MgCl2 (1.5 mM at 1X) and dNTPs (0.2 mM each at 1X). Additional MgCl2(25 mM) is supplied separately for optimization.
Quality
Each batch of KAPA3G Plant DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA3G Plant PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- KAPA3G Plant DNA Polymerase 2.5 U/μL
- KAPA Plant PCR Buffer (2X) contains MgCl2 and dNTPs
- MgCl2 25 mM
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Articles
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
Protocols
Robust Taq DNA polymerase reagents and PCR kits for efficient extraction, purification, and amplification of routine and challenging DNA template sequences.
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