11988506001
Roche
Anti-HA-Fluorescein, High Affinity
from rat IgG1
Synonym(s):
antibody
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About This Item
UNSPSC Code:
12352200
Recommended Products
biological source
rat
conjugate
fluorescein conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F10, monoclonal
form
lyophilized
packaging
pkg of 25 μg
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
storage temp.
−20°C
General description
Anti-HA-Fluorescein, High Affinity is a monoclonal antibody for the highly sensitive detection of HA-tagged recombinant proteins, Fab fragments, conjugated to fluorescein.
Anti-HA-Fluorescein, High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the human hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".
Contents
Lyophilizate, stabilized
Anti-HA-Fluorescein, High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the human hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".
Contents
Lyophilizate, stabilized
Specificity
Anti-HA-Fluorescein, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
Immunogen
Amino acids 98 to 106 from the human influenza virus hemagglutinin protein
Application
Anti-HA-Fluorescein, High Affinity has been used in immunofluorescence labeling of chinese hamster ovary live cells and in immunocytochemistry of neuron cell culture.
Quality
The Anti-HA-Fluorescein, High Affinity antibody is function tested by flow cytometry analysis of a HA-tagged fusion protein
Preparation Note
Stabilizers: proteinous stabilizers
Working concentration: Flow cytometry: 1 to 5 μg/ml
Immunofluorescence: 1 to 5 μg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Storage conditions (working solution): Reconstituted antibody is stable at 2 to 8 °C for two months. Alternatively store aliquots at -15 to -25 °C for up to six months.
Note: Avoid thawing and repeated freezing.
Working concentration: Flow cytometry: 1 to 5 μg/ml
Immunofluorescence: 1 to 5 μg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Storage conditions (working solution): Reconstituted antibody is stable at 2 to 8 °C for two months. Alternatively store aliquots at -15 to -25 °C for up to six months.
Note: Avoid thawing and repeated freezing.
Reconstitution
Add 0.5 ml double-distilled water to a final concentration of 50 μg/ml. Rehydrate for 10 minutes prior to use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
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