11742027001
Roche
α-Glucosidase Assay
suitable for activity assay, kit of 1 (5 components)
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Synonym(s):
α-Glucosidase, Alpha-glucosidase activity assay kit, Maltase, glucosidase, glycosidase, neutral alpha-glucosidase (NAG)
UNSPSC Code:
12161503
Recommended Products
usage
sufficient for 30 tests (and 10 blanks)
species reactivity
human
packaging
kit of 1 (5 components)
manufacturer/tradename
Roche
storage condition
(Keep container tightly closed in a dry and well-ventilated place.)
technique(s)
activity assay: suitable
detection method
colorimetric
storage temp.
2-8°C
Related Categories
General description
Colorimetric assay for the quantitative determination of α-glucosidase (maltase) in human semen research samples. α-Glucosidase is a key enzyme involved in carbohydrate hydrolysis. It is essential for the conversion of oligosaccharides and disaccharides into glucose.
Application
α-Glucosidase Assay Kit has been used to measure neutral α-glucosidase (NAG) activity in seminal samples for the evaluation of epididymal function.
Features and Benefits
- Fast and sensitive: The analysis can be performed using only 15 μl of semen or seminal plasma, ensuring accurate results while minimizing the sample volume needed.
- Specific: The assay is designed to measure the activity of neutral α-glucosidase in human semen samples in life science research applications. It is not designed for diagnostic applications and cannot be used to measure α-glucosidase in other specimens.
- Compatible: The assay is compatible with microplate assay formats and standard cuvette formats
Packaging
1 kit containing 5 components.
Components
The α-Glucosidase Assay contains the substrate and all buffers for the measurement of α-glucosidase in human semen or seminal plasma samples. It also contains a standard substance to set up a standard curve.
Preparation Note
Storage conditions (working solution): For stability of reconstituted kit components see section "General considerations".
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Reaction Buffer
- Substrate Solution containing 4-nitrophenyl-α-D-glucopyranoside
- Stopping Buffer
- Castanospermine
- Standard Solution containing 4-nitrophenol
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
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Certificates of Analysis (COA)
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Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Emerging strategies for the activity assay and inhibitor screening of alpha-glucosidase
Zhang X, et al.
Food & Function, 11(1), 66-82 (2020)
Fatima Kazue Okada et al.
Reproduction & fertility, 1(1), 1-10 (2020-10-09)
According to the World Health Organization guidelines, ejaculatory abstinence (EA) of 2-7 days is recommended for semen analysis. This study aimed to determine how seminal quality may be affected by two EA periods from the same man. Seminal samples from
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