11699105001
Roche
PCR Buffer Without MgCl2, 10x concentrated
solution, pkg of 3 × 1 mL
Synonym(s):
buffer
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About This Item
UNSPSC Code:
41105600
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General description
The PCR buffer without MgCl2, in combination with the MgCl2 stock solution, is used for the individual adjustment of the Mg2+ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg2+ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl2 stock solution.
Application
The PCR buffer without MgCl2 has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).
Physical form
Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)
Other Notes
For general laboratory use.
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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