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11681451001

Roche

NBT/BCIP Stock Solution

Synonym(s):

5-bromo-4-chloro-3-indoyl phosphate, BCIP

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10 X 1 L
CN¥1,325.92

CN¥1,325.92

Available to ship onApril 16, 2025Details

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10 X 1 L
CN¥1,325.92

About This Item

UNSPSC Code:
12352204

CN¥1,325.92

Available to ship onApril 16, 2025Details

form

solution

Quality Level

100

mol wt

817.7

packaging

pkg of 8 mL

manufacturer/tradename

Roche

storage temp.

2-8°C

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This Item
M3769M2154M7528
Sigma-Aldrich

M2520

Medium 199

Sigma-Aldrich

M3769

Medium 199

Sigma-Aldrich

M2154

Medium 199

Medium 199 HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

M7528

Medium 199

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM

components

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no

components

sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
Earle’s salts (5% CO2): yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

400

Quality Level

400

General description

The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N

Evaluation: visually

Application

  • The solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, including: Southern blot
  • Northern blot
  • Western blot[1]
  • Colony and plaque lifts
  • In situ hybridization[2][3]
  • and for applications in immunohistochemistry and immunocytochemistry.
Note: NBT/BCIP may be used with both nitrocellulose and nylon membranes.

Physical form

Solution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in 67% (DMSO) (v/v).
Note: The color of the solution can vary from yellowish to light brown.

Preparation Note

Working solution: Preparation of staining solution
Always prepare this solution fresh shortly before use.
  • For all applications except DIG System: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
  • For DIG System applications: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
Note: Do not include MgCl2 in the detection buffer as this may lead to spotty background on the membrane after the detection procedure. Insoluble in water.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    60011320
    46079521
    46079520
    34527921
    34527920

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    Jaroslav Fabik et al.
    Biology open, 9(6) (2020-07-04)
    Cranial neural crest cells (cNCCs) originate in the anterior neural tube and populate pharyngeal arches in which they contribute to formation of bone and cartilage. This cell population also provides molecular signals for the development of tissues of non-neural crest
    João L Q Durigan et al.
    Muscle & nerve, 49(1), 120-128 (2013-04-30)
    Neuromuscular electrical stimulation (NMES) is used to improve quadriceps mass after anterior cruciate ligament (ACL) injury. We studied the effect of NMES on mRNA levels of atrophy genes in the quadriceps muscle of rats after ACL transection. mRNA levels of
    Garth R Ilsley et al.
    Scientific reports, 10(1), 4961-4961 (2020-03-20)
    Single-cell RNA-seq has been established as a reliable and accessible technique enabling new types of analyses, such as identifying cell types and studying spatial and temporal gene expression variation and change at single-cell resolution. Recently, single-cell RNA-seq has been applied
    Morgan Haugen et al.
    Cold Spring Harbor protocols, 2010(10), pdb-pdb (2010-10-05)
    Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have
    Masoud Nasri et al.
    Haematologica, 105(3), 598-609 (2019-06-30)
    A Autosomal-dominant ELANE mutations are the most common cause of severe congenital neutropenia. Although the majority of congenital neutropenia patients respond to daily granulocyte colony stimulating factor, approximately 15 % do not respond to this cytokine at doses up to
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    Protocols

    NBT/BCIP Stock Solution Protocol & Troubleshooting

    NBT/BCIP Stock Solution Protocol & Troubleshooting

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