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Roche

Fluorescein-12-dUTP

≥85% (HPLC), suitable for ELISA, suitable for hybridization, solution

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Synonym(s):
Fluorescein-12-dUTP, dUTP
UNSPSC Code:
41116100

Quality Level

100

Assay

≥85% (HPLC)

form

solution

mol wt

Mr 1018.4 (fluorescein-12-dUTP-Li4)

packaging

pkg of 25 μL (25 nmol; 1mM)

manufacturer/tradename

Roche

technique(s)

ELISA: suitable
hybridization: suitable

storage temp.

−20°C

Related Categories

Application

Fluorescein-12-dUTP is used as a substrate for:
  • terminal transferase
  • DNA polymerase I (holoenzyme and Klenow fragment),
  • Taq DNA polymerase
  • reverse transcriptase (e.g., from AMV and M-MuLV).
Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.

Quality

Typical analysis: At least 85% Fluorescein-12-dUTP (HPLC, area%).

Physical form

Fluorescein-12-dUTP, tetralithium salt.

Storage and Stability

The unopened reagent is stable at 15 to 25°C until the expiration date printed on the label. Decomposition of approx. 5% may occur within 6 months.

Other Notes

For life science research only. Not for use in diagnostic procedures.

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

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