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Merck
CN

11363905910

Roche

Digoxigenin-11-ddUTP

solution, =85% (HPLC), pkg of 25 μL (25 nmol; 1mM)

Synonym(s):

digoxigenin

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About This Item

UNSPSC Code:
41116100
NACRES:
NA.54
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Product Name

Digoxigenin-11-ddUTP, solution, =85% (HPLC), pkg of 25 μL (25 nmol; 1mM)

Quality Level

assay

85% (HPLC)

form

solution

mol wt

(Molecular weight: Mr = 1074.7 (DIG-11-ddUTP-Li4))

packaging

pkg of 25 μL (25 nmol; 1mM)

manufacturer/tradename

Roche

storage temp.

−20°C

Analysis Note

Typical analysis: 85% DIG-11-ddUTP (HPLC, area%)
Function tetsted by end labeling.

Formula: C43H61N4O20P3Li4

Application

Digoxigenin-11-ddUTP has been used:
  • for labeling of DNA fragments in linker histone binding assay
  • for end labeling of NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) for electrophoretic motility shift assay and labelling of ATP citrate lyase gene for gel shift assay

for end labeling of DNA for fragmentation reactions

General description

Digoxigenin-11-ddUTP is a 1 mM solution of the tetralithium salt. Digoxigenin-11-ddUTP is a component of probe used for end labeling. Digoxigenin-11-ddUTP is an alternative to classical radioactive labeling for electrophoretic mobility shift assay and is highly stable compared to radioactive probes.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

does not flash

flash_point_c

does not flash


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A sequence-based variation map of 8.27 million SNPs in inbred mouse strains
Frazer KA, et al.
Nature, 448(7157), 1050-1050 (2007)
Non-radioactive electrophoretic mobility shift assay using digoxigenin-ddUTP labeled probes
Kass J, et al.
Drosophila Information Service, 83, 185-188 (2000)
Inhibition of extracellular signal-regulated kinases 1/2 provides neuroprotection in spinal cord ischemia/reperfusion injury in rats: relationship with the nuclear factor-kappaB-regulated anti-apoptotic mechanisms
Lu K, et al.
Journal of Neurochemistry, 114(1), 237-246 (2010)
J Maupin-Furlow et al.
Journal of bacteriology, 178(2), 340-346 (1996-01-01)
The CO dehydrogenase enzyme complex from Methanosarcina thermophila contains a corrinoid/iron-sulfur enzyme composed of two subunits (delta and gamma). The cdhD and cdhE genes, which encode the delta and gamma subunits, respectively, were cloned and sequenced. The cdhD gene is
HILS1 is a spermatid-specific linker histone H1-like protein implicated in chromatin remodeling during mammalian spermiogenesis
Yan W, et al.
Proceedings of the National Academy of Sciences of the USA, 100(18), 10546-10551 (2003)

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