11363514910
Roche
DIG Luminescent Detection Kit
sufficient for 50 blots (10 cm x 10 cm each), kit of 1 (5 components), suitable for hybridization
Synonym(s):
luminescent detection kit
Sign Into View Organizational & Contract Pricing
All Photos(3)
About This Item
UNSPSC Code:
41116134
Assay
>98% (NMR)
Quality Level
usage
sufficient for 50 blots (10 cm x 10 cm each)
packaging
kit of 1 (5 components)
manufacturer/tradename
Roche
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
hybridization: suitable
greener alternative category
, Aligned
storage temp.
−20°C
General description
DIG Luminescent Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay with luminescence on nylon membranes. The nonradioactive DIG system uses digoxigenin, a steroid hapten, coupled to deoxyuridine triphosphate (dUTP), UTP or didUTP to label DNA, RNA or oligonucleotides for hybridization and subsequent luminescent detection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application
DIG Luminescent Detection Kit has been used for highly sensitive and specific detection of DIG-labeled nucleic acids in:
- critical commercial assays
- Southern hybridization
- northern analysis
- plaque or colony lifts with anti-DIG-AP conjugate and the chemiluminescent substrate CSPD. Chemiluminescent detection with CSPD (following the instructions of the kit) is as sensitive as radioactive methods, but requires much shorter exposure times.
Packaging
1 kit containing 5 components.
Specifications
Sensitivity: 0.03 pg of homologous DNA or 0.1 pg of homologous RNA is detected in a Southern or dot blot after <30-min exposure time. Single-copy genes are detected in 0.3 μg mammalian genomic DNA.
Preparation Note
Working concentration: 0.5mg/ml for flow cytometry
Enzymatic dephosphorylation of the dioxetane CSPD by alkaline phosphatase leads to the metastable phenolate anion, which decomposes and emits light at 477nm. The light emission increases with time until a constant intensity is attained.
Assay Time
Note: Store protected from light.
- Storage conditions (working solution): Antibody conjugate (vial 3): once opened, should be stored at 2 to 8°C
- Blocking Reagent (bottle 4): in solution at 2 to 8°C
- CSPD (vial 5): at 2 to 8°C when frequently used. Repeated freeze/thaw cycles should be avoided.
Enzymatic dephosphorylation of the dioxetane CSPD by alkaline phosphatase leads to the metastable phenolate anion, which decomposes and emits light at 477nm. The light emission increases with time until a constant intensity is attained.
Assay Time
- Immunological detection 1.5 hours
- Signal detection 5 to 30 minute
Note: Store protected from light.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- DIG-labeled Control DNA 5 µg/ml
- DNA Dilution Buffer
- Anti-digoxigenin-AP antibody, Fab fragments 750 U/ml
- Blocking Reagent
- CSPD 11.6 mg/ml
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
does not flashNot applicable
Flash Point(C)
does not flashNot applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Dongqing Huang et al.
Genetics, 203(1), 353-368 (2016-03-28)
In response to replication stress, a phospho-signaling cascade is activated and required for coordination of DNA repair and replication of damaged templates (intra-S-phase checkpoint) . How phospho-signaling coordinates the DNA replication stress response is largely unknown. We employed state-of-the-art liquid
Karl Dichtl et al.
Molecular microbiology, 95(3), 458-471 (2014-11-27)
Echinocandins inhibit β-1,3-glucan synthesis and are one of the few antimycotic drug classes effective against Aspergillus spp. In this study, we characterized the β-1,3-glucan synthase Fks1 of Aspergillus fumigatus, the putative target of echinocandins. Data obtained with a conditional mutant
Fang Zheng et al.
Nature communications, 12(1), 1341-1341 (2021-02-28)
Hypoxia-inducible factor-1 (HIF-1) is a master driver of glucose metabolism in cancer cells. Here, we demonstrate that a HIF-1α anti-sense lncRNA, HIFAL, is essential for maintaining and enhancing HIF-1α-mediated transactivation and glycolysis. Mechanistically, HIFAL recruits prolyl hydroxylase 3 (PHD3) to
Nannan Zhang et al.
Current protocols in immunology, 126(1), e78-e78 (2019-09-05)
MicroRNAs (miRNAs) are short (19- to 25-nucleotide) noncoding RNA molecules that target mRNAs to repress gene expression and that play important roles in regulating many fundamental biological functions including cell differentiation, development, growth, and metabolism. They are well conserved in
DNA Replication Stress Phosphoproteome Profiles Reveal Novel Functional Phosphorylation Sites on Xrs2 in Saccharomyces cerevisiae.
Huang D, et al.
Genetics, 203(1), 353-368 (2016)
Articles
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service