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11348639001

Sigma-Aldrich

Propidium iodide

96% (TLC), solution, 0.5 mg/mL, suitable for hematology, histology

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About This Item

UNSPSC Code:
12171500

Assay

96% (TLC)

form

solution

packaging

pkg of 20 mL (0.5mg/mL)

manufacturer/tradename

Roche

concentration

0.5 mg/mL

pH

7.0

εmax

6000 at 493 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

General description

Propidium iodide is a fluorogenic compound. It is used in nuclear staining as it binds to nucleic acids stoichiometrically.
Stain for non-viable cells or DNA counterstain in flow cytometric studies.
Emission maximum: 632nm
Excitation maximum: 493nm

Application

Propidium iodide is used for:
  • Discrimination of viable and non-viable cells in flow cytometry
  • Determination of DNA content in flow cytometry

Physical form

Solution, red/orange, containing 0.5mg/mL propidium iodide in phosphate-buffered saline (pH approx. 7.4) with 0.09% sodium azide.

Preparation Note

Working concentration: 0.5mg/mL for flow cytometry

Other Notes

For life science research only. Not for use in diagnostic procedures.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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