11332465001
TWEEN® 20
non-ionic, aqueous solution, 10% (w/v)
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Synonym(s):
TWEEN 20, Tween, Tween 20
UNSPSC Code:
12352200
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description
Formula: C58H114O26, for n = 20
form
solution
mol wt
Mr 1228 (for n= 20)
packaging
pkg of 5 × 10 mL
manufacturer/tradename
Roche
technique(s)
cell culture | plant: suitable
microbiological culture: suitable
CMC
~0.06 mM
absorption
= 0.025 at 254 nm at 0.05 % (w/v)
shipped in
wet ice
storage temp.
2-8°C
General description
Tween 20, also referred to as poly(oxyethylene)n-sorbitan-monolaurate, is a non-ionic surfactant. It is mainly used in membrane research.
Application
Tween 20 is used for:
- solubilizing membrane proteins during isolation of membrane-protein complexes.
- a subsequent protein detection by UV light absorption, due to its low UV absorbance.
- ELISA (enzyme-linked immunosorbent assay) and immunoblots.
Quality
Purity: <2 ppm peroxide content (as H2O2), <200 μM carbonyls, <100 μS conductivity. Specially purified for membrane research.
Physical form
10% (w/v) aqueous solution under nitrogen, supplied in injection glass bottles.
Preparation Note
Working concentration: >10 mg/mg membrane lipid
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
TWEEN is a registered trademark of Croda International PLC
WGK
WGK 1
Flash Point(F)
No data available
Flash Point(C)
No data available
Certificates of Analysis (COA)
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Danny K Chou et al.
Journal of pharmaceutical sciences, 94(6), 1368-1381 (2005-04-29)
The objectives of this work were to determine the effects of nonionic surfactants (Tween 20 and Tween 80) on agitation-induced aggregation of the recombinant fusion protein, Albutropintrade mark (human growth hormone genetically fused to human albumin), and to characterize the
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