11277065910
Roche
DIG DNA Labeling Mix
Synonym(s):
DNA labeling
About This Item
form
solution
Quality Level
usage
sufficient for 25 standard reactions
packaging
pkg of 50 μL
manufacturer/tradename
Roche
greener alternative product characteristics
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sustainability
Greener Alternative Product
color
colorless
solubility
water: miscible
greener alternative category
storage temp.
−20°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application
- Southern blots
- northern blots
- dot blots
- screening of gene libraries
- in situ hybridizations
Features and Benefits
10x concentrated dNTP labeling mixture: 1mM dATP, 1mM dCTP, 1mM dGTP, 0.65mM dTTP, 0.35mM DIG-dUTP, alkali-labile, pH 7.5 (+20°C)
Assay Time: Labeling: 1 hour to O/N
Labeling efficiency: With 1μg DNA per assay, approx. 10% of the nucleotides are incorporated into about 250ng of newly synthesized labeled DNA within 1 hour and approx. 30% of the nucleotides into about 750ng after 20 hours.
Quality
Principle
Preparation Note
As template for the labeling reaction
- DNA fragments of at least 100bp
- Linearized plasmids, cosmid or λDNA,
- Supercoiled DNA,
- Or minimal amounts of DNA (10ng), e.g., DNA restriction fragments isolated from low melting point agarose can be used.
Note: Linear DNA is labeled more efficiently than circular and supercoiled DNA.
Other Notes
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
No data available
Flash Point(C)
No data available
Certificates of Analysis (COA)
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Articles
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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