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Roche

DIG DNA Labeling Mix

greener alternative

Synonym(s):

DNA labeling

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About This Item

UNSPSC Code:
41105500

form

solution

Quality Level

usage

sufficient for 25 standard reactions

packaging

pkg of 50 μL

manufacturer/tradename

Roche

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

color

colorless

solubility

water: miscible

greener alternative category

storage temp.

−20°C

General description

DIG DNA Labeling Mix is an easy-to-use labeling mixture for rapid random-primed labeling with digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP). DIG-dUTP is incorporated every 20 to 25 nucleotides in the newly synthesized DNA. This density of haptens in the DNA yields the highest sensitivity in the detection reaction.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

The DIG DNA labeling mix has been used in a variety of hybridization techniques including:
  • Southern blots
  • northern blots
  • dot blots
  • screening of gene libraries
  • in situ hybridizations

Features and Benefits

Contents
10x concentrated dNTP labeling mixture: 1mM dATP, 1mM dCTP, 1mM dGTP, 0.65mM dTTP, 0.35mM DIG-dUTP, alkali-labile, pH 7.5 (+20°C)

Assay Time: Labeling: 1 hour to O/N
Labeling efficiency: With 1μg DNA per assay, approx. 10% of the nucleotides are incorporated into about 250ng of newly synthesized labeled DNA within 1 hour and approx. 30% of the nucleotides into about 750ng after 20 hours.

Quality

Function-tested in the DIG DNA Labeling Kit and in the DIG Nucleic Acid Detection Kit.

Principle

DIG-labeled DNA probes are generated according to the random-primed labeling method which is based on the hybridization of random oligonucleotides to the denatured DNA template. The complementary DNA strand is synthesized by Klenow enzyme which uses the 3′-OH termini of the random oligonucleotides as primers and a mixture of deoxyribonucleotides containing DIG-11-dUTP, alkali-labile, for elongation. DIG-dUTP is incorporated every 20 to 25 nucleotides in the newly synthesized DNA. This density of haptens in the DNA yields the highest sensitivity in the detection reaction.

Preparation Note

Sample Materials
As template for the labeling reaction

  • DNA fragments of at least 100bp
  • Linearized plasmids, cosmid or λDNA,
  • Supercoiled DNA,
  • Or minimal amounts of DNA (10ng), e.g., DNA restriction fragments isolated from low melting point agarose can be used.

Note: Linear DNA is labeled more efficiently than circular and supercoiled DNA.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Gerald Losensky et al.
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It was recently shown that haloarchaeal strains of different genera are able to adhere to surfaces and form surface-attached biofilms. However, the surface structures mediating the adhesion were still unknown. We have identified a novel surface structure with Halobacterium salinarum
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Developmental biology, 459(2), 161-180 (2019-12-22)
Animal embryogenesis is initiated by maternal factors, but zygotic genome activation (ZGA) shifts regulatory control to the embryo during blastula stages. ZGA is thought to be mediated by maternally provided transcription factors (TFs), but few such TFs have been identified
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<BIG>Okada R, et al.</BIG>
The Journal of Comparative Neurology, 514 (2009)
Diane M Ramos et al.
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Precise temporal and spatial regulation of transgene expression is a critical tool to investigate gene function in developing organisms. The most commonly used technique to achieve tight control of transgene expression, however, requires the use of specific DNA enhancers that

Articles

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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