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Roche

DNA Molecular Weight Marker VI, DIG-labeled

greener alternative

solution, pkg of 500 μL (10 μg/ml)

Synonym(s):

DNA marker

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About This Item

UNSPSC Code:
41105335

form

solution

Quality Level

100

packaging

pkg of 500 μL (10 μg/ml)

manufacturer/tradename

Roche

greener alternative product characteristics

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Aligned,

storage temp.

−20°C

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General description

DNA Molecular Weight Marker VI, DIG-labeled is a fragment mixture prepared by cleavage of pBR328 DNA and pBR328 DNA with restriction endonucleases Bgl I and Hinf I respectively. The size of the fragment ranges from 0.15 to 2.1 kbp.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DNA Molecular Weight Marker VI, DIG-labeled, has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection. It has also been used as a size standard for amplified DNA.

Components

Ready-to-use solutions in 10mM Tris-HCl, 1mM EDTA, pH 8.0.

Sequence

The mixture contains 12 fragments wit the following base pair lengths: 154/154, 220, 234/234, 298/298, 394, 453, 517, 653, 1033, 1230, 1766, and 2176 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Other Notes

12 fragments: 154/154; 220; 234/234; 298/298; 394; 453; 517; 653; 1,033; 1,230; 1,766; 2,176 bp
Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available

Regulatory Information

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M Agüero et al.
Journal of clinical microbiology, 41(9), 4431-4434 (2003-09-06)
This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A
Stéphanie Tomé et al.
PLoS currents, 6, doi:10-doi:10 (2014-03-13)
Many human diseases are associated with the abnormal expansion of unstable trinucleotide repeat sequences. The mechanisms of trinucleotide repeat size mutation have not been fully dissected, and their understanding must be grounded on the detailed analysis of repeat size distributions
R Ferretti et al.
Applied and environmental microbiology, 67(2), 977-978 (2001-02-07)
A PCR-based method for the detection of Salmonella spp. in food was developed. The method, set up on typical salami from the Italian region of Marche, is sensitive and specific and shows excellent correlation with the conventional method of reference

Articles

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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