Product Name
GTP, =98% (HPLC), solution, pkg of 400 μL (100 mM; 40 μmol)
description
Lithium salt
assay
98% (HPLC)
form
solution
packaging
pkg of 400 μL (100 mM; 40 μmol)
manufacturer/tradename
Roche
storage temp.
−20°C
Application
GTP, lithium salt, is suitable for applications such as in vitro RNA transcription.
GTP has been used in cell-free generation of COPII-coated procollagen I carriers and to label transcription elongation complexes.
General description
GTP is a 100 mM solution of the lithium salt (pH 7).
Guanosine triphosphate (GTP) is a purine nucleoside triphosphate. This nucleotide is mostly used as substrate in RNA synthesis during transcription. It is comprised of a ribose sugar and three phosphates with nucleobase and triphosphate moiety attached to 1′and 5′ carbons. It may also be involved in signal transduction, energy transfer within the cell, as an activator of substrates in metabolic reaction or for protein synthesis and gluconeogenesis.
Other Notes
1 mM GTP = 13.7 A252 units
For life science research only. Not for use in diagnostic procedures.
Analysis Note
Typical analysis:
- ≥98% GTP (HPLC), ≤1.5% GDP (HPLC), ≤0.5% GMP (HPLC).
- Tested for the absence of RNases according to the current Quality Control procedures.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Mfd Dynamically Regulates Transcription via a Release and Catch-Up Mechanism
Le TT, et al.
Cell (2017)
Cell-free Generation of COPII-coated Procollagen I Carriers
Yuan L, et al.
Bio-protocol, 7(22) (2017)
Youngseo Cheon et al.
Genome biology, 22(1), 294-294 (2021-10-20)
Promoter-proximal pausing of RNA polymerase II (RNAPII) is a critical step for the precise regulation of gene expression. Despite the apparent close relationship between promoter-proximal pausing and nucleosome, the role of chromatin remodeler governing this step has mainly remained elusive.
Riku Nagai et al.
Methods and protocols, 4(3) (2021-07-22)
The rates of translation elongation or termination in eukaryotes are modulated through cooperative molecular interactions involving mRNA, the ribosome, aminoacyl- and nascent polypeptidyl-tRNAs, and translation factors. To investigate the molecular mechanisms underlying these processes, we developed an in vitro translation
Related Content
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service