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Merck
CN

11112481001

Roche

Streptavidin-β-Gal conjugate

lyophilized (clear, colorless solution after reconstitution), pkg of 500 U

Synonym(s):

streptavidin

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About This Item

UNSPSC Code:
12352200
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lyophilized (clear, colorless solution after reconstitution)

packaging

pkg of 500 U

manufacturer/tradename

Roche

storage temp.

−20°C

General description

Streptavidin is covalently coupled to β-galactosidase from E. coli.

Application

  • Use Streptavidin-β-Gal to detect biotinylated compounds using: ELISA
  • Immunohistocytochemistry
  • Western blot
  • In situ hybidization

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: 100 to 500 mU/ml
  • Immunohistocytochemistry: 250 to 500 mU/ml
  • Western blot: 250 to 500 mU/ml


Storage conditions (working solution): The reconstituted solution is stable at 2 to 8 °C for four weeks. Aliquots should be stored at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Add 1 ml double-distilled water to a final concentration of 500 U/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

11 - Combustible Solids

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WGK 2

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Regulatory Information

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Ramona Trebbien et al.
Virology journal, 8, 434-434 (2011-09-10)
Pigs are considered susceptible to influenza A virus infections from different host origins because earlier studies have shown that they have receptors for both avian (sialic acid-alpha-2,3-terminal saccharides (SA-alpha-2,3)) and swine/human (SA-alpha-2,6) influenza viruses in the upper respiratory tract. Furthermore
Monica Ronco et al.
Developmental biology, 313(1), 80-92 (2007-12-07)
Our understanding of the developmental mechanisms underlying the vast diversity of arthropod appendages largely rests on the peculiar case of the dipteran Drosophila melanogaster. In this insect, homothorax (hth) and extradenticle (exd) together play a pivotal role in appendage patterning

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