11074547001
Roche
Insulin-Transferrin-Sodium Selenite Supplement
suitable for cell culture, lyophilized, pkg of 50 mg (for 5 l medium)
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Synonym(s):
ITS
UNSPSC Code:
12352207
Recommended Products
biological source
human serum (transferrin)
synthetic (sodium selenite)
Quality Level
recombinant
expressed in human cells
sterility
non-sterile; 0.2 μm filtered
Assay
>98% (Transferrin, SDS-PAGE)
>99% (Insulin, HPLC)
>99% (Sodium selenite)
form
lyophilized
packaging
pkg of 50 mg (for 5 l medium)
manufacturer/tradename
Roche
technique(s)
cell culture | mammalian: suitable
solubility
water: miscible
storage temp.
2-8°C
Gene Information
bovine ... INS(280829)
human ... TFRC(7037)
Related Categories
General description
The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio.
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic
The insulin-transferrin-sodium selenite (ITS) supplement comprises three of the most crucial growth factors for many cell types. Insulin is a component of serum-free media formulations for all primary cells and cell lines. It stimulates cell growth, increases fatty acid and glycogen synthesis in a serum-free medium. Transferrin is an essential growth factor for many cell types. Selenium is very often necessary for optimal cell growth. ITS permits an appreciable reduction in serum requirements for cellular growth. ITS is also an antioxidant that supports the growth of in vivo derived (IVD) embryos. Insulin promotes embryonic growth and metabolism. The iron transport in the embryo is mediated by transferrin. Sodium selenite protects from oxidative damage and inhibits lipid peroxidation. ITS is useful in human chondrocytes monolayer culture.
Application
The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added.
Preparation Note
Working concentration: Insulin, 5 μg/ml, transferrin, 5 μg/ml, sodium selenite, 5 ng/ml (3.0 x 10-8 M)
Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.
Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.
Reconstitution
The insulin-transferrin-sodium selenite supplement should be reconstituted in 5 ml or 25 ml sterile double-dist. water (1000 x concentrated stock solution).
Further dilution with culture medium.
Further dilution with culture medium.
Other Notes
For life science research only. Not for use in diagnostic procedures.
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
常规特殊物品
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K H Chua et al.
European cells & materials, 9, 58-67 (2005-06-18)
This study was to investigate the effects of insulin-transferrin-selenium (ITS) on the proliferation and quantitative gene expression of adult human nasal septum chondrocytes in monolayer culture expansion and the formation of tissue engineered hyaline cartilage. Effects of ITS on human
Imran Khan et al.
Theriogenology, 152, 147-155 (2020-05-16)
Hypothermic storage of gametes and embryos at 4 °C can be used as an alternative to cryopreservation, but hypothermic preservation can maintain embryo viability for a short duration only. This study investigated the effect of insulin-transferrin-sodium selenite (ITS) in embryo culture
Jyoti Motwani et al.
Epigenomics, 13(8), 577-598 (2021-03-31)
Aims & objectives: The aim of this study was to investigate the role of DNA methylation in invasiveness in melanoma cells. Materials & methods: The authors carried out genome-wide transcriptome (RNA sequencing) and reduced representation bisulfite sequencing methylome profiling between noninvasive (n = 4)
María Virumbrales-Muñoz et al.
Lab on a chip, 20(23), 4420-4432 (2020-10-27)
Clear cell renal cell carcinoma (ccRCC) is a common genitourinary cancer associated with the development of abnormal tumor angiogenesis. Although multiple anti-angiogenic therapies have been developed, responses to individual treatment are highly variable between patients. Thus, the use of one-patient
Jane Ying-Chieh Lee et al.
Frontiers in pharmacology, 7, 81-81 (2016-04-12)
SAHA is a class I HDAC/HDAC6 co-inhibitor and an autophagy inducer currently undergoing clinical investigations in breast cancer patients. However, the molecular mechanism of action of SAHA in breast cancer cells remains unclear. In this study, we found that SAHA
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