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11051466001

Roche

dGTP

Name: dGTP
Brand: ROCHE

=98%, solution, 100 mM, suitable for PCR, suitable for DNA sequencing

Synonym(s):

2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, 2′-deoxy-guanosine-5′-triphosphate, deoxy-guanosine-5′-triphosphate, 2′-, dGTP-Na₃

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About This Item

Empirical Formula (Hill Notation):

C₁₀H₁₃N₅Na₃O₁₃P₃

CAS Number:

93919-41-6

Molecular Weight:

573.13

MDL number:

MFCD00080337

UNSPSC Code:

41116100

PubChem Substance ID:

329749301

Quality Level

100

description

Lithium salt

Assay

98%

form

solution

usage

sufficient for 6,250 standard reactions (PCR)

packaging

pkg of 250 μL (25 μmol; 100 mM)

manufacturer/tradename

Roche

concentration

100 mM

technique(s)

DNA amplification: suitable
DNA sequencing: suitable
DNA synthesis: suitable
PCR: suitable

storage temp.

−20°C

SMILES string

[Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1

InChI

1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1

InChI key

IWGGLKOTEOCWQP-BIHLCPNHSA-K

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Related Categories

PCR Reagents & Kits

Roche Life Science Products

General description

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.

dGTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).
dGTP solution in water (pH 7), 100 mM, lithium salt.

Application

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.

Use dGTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

Quality

Typical analysis:

≥98% dGTP (HPLC), ≤1.5% dGDP (HPLC), ≤0.5% dGMP (HPLC)
Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀
DNase activity negative, according to the current Quality Control procedures
Suitability for PCR

Unit Definition

1 μmol dGTP = 13.7 A₂₅₂ units

Other Notes

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:

nucleotide sets (all 4 nucleotides in separate vials)
ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:

kits (dNTPs, buffer, and polymerase in separate vials)
ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)