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Merck
CN

11051440001

Roche

dATP

=98%, solution, 100 mM, suitable for DNA sequencing, suitable for DNA amplification

Synonym(s):

2′-Deoxyadenosine 5′-triphosphate sodium salt solution, 2′-deoxy-adenosine-5′-triphosphate, deoxy-adenosine-5′-triphosphate, 2′-, dATP

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About This Item

UNSPSC Code:
41116100
NACRES:
NA.54

Key Documents

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Product Name

dATP, =98%, solution, 100 mM, suitable for DNA sequencing, suitable for DNA amplification

InChI

1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/q;+1/p-1/t5-,6+,7+;/m0./s1

InChI key

YJWCICGGRLOGEH-VWZUFWLJSA-M

SMILES string

[Na+].Nc1ncnc2n(cnc12)[C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)O3

description

Lithium salt

assay

98%

form

solution

usage

sufficient for 6,250 standard reactions (PCR)

packaging

pkg of 250 μL (25 μmol; 100 mM)

manufacturer/tradename

Roche

concentration

100 mM

technique(s)

DNA amplification: suitable
DNA sequencing: suitable
DNA synthesis: suitable
PCR: suitable

storage temp.

−20°C

Quality Level

100

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Analysis Note

Typical analysis:
  • ≤98% dATP (HPLC), ≤1.5% dADP (HPLC), ≤0.5% dAMP (HPLC)
  • Absorbance determination A250/A260, A280/A260, A290/A260
  • DNase activity negative, according to the current Quality Control procedures
  • Suitability for PCR

Application

2′-Deoxyadenosine 5′-triphosphate sodium salt solution is used in DNA sysnthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.
Use dATP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
dATP has been used to resume transcription.

Biochem/physiol Actions

Deoxyadenosine-5-triphosphate (dATP) plays a major role in the initiation of apoptosis in vitro. dATP is considered as an inhibitor of mammalian ribonucleotide reductase, thereby DNA synthesis.

General description

2′-Deoxyadenosine 5′-triphosphate (dATP) is made up of a nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar bound to a chain of three phosphate residues. dATP is used by cells for synthesis of DNA by DNA polymerases.
dATP is a 100 mM clear, colorless solution of the lithium salt (pH 7).Download the CofA, SDS and IFU s

Other Notes

1 μmol dATP = 15.0 A260 units
For life science research only. Not for use in diagnostic procedures.
Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
  • nucleotide sets (all 4 nucleotides in separate vials)
  • ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)
They are also vital components of:
  • kits (dNTPs, buffer, and polymerase in separate vials)
  • ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

No data available

flash_point_c

No data available

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Certificates of Analysis (COA)

Lot/Batch Number
47257123
47257122
47257121
47257120
14264352

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Mfd Dynamically Regulates Transcription via a Release and Catch-Up Mechanism
Le TT, et al.
Cell (2018)
Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c
Liu X, et al.
Cell, 86(1), 147-157 (1996)
Stable suppression of the R2 subunit of ribonucleotide reductase by R2-targeted short interference RNA sensitizes p53(-/-) HCT-116 colon cancer cells to DNA-damaging agents and ribonucleotide reductase inhibitors
Lin ZP, et al.
The Journal of Biological Chemistry, 279(26), 27030-27038 (2004)

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