10821705001
Roche
DNA Molecular Weight Marker V
pkg of 50 μg (in 200 μl), solution
Synonym(s):
DNA Marker
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About This Item
UNSPSC Code:
41105335
Recommended Products
form
solution
packaging
pkg of 50 μg (in 200 μl)
manufacturer/tradename
Roche
storage condition
avoid repeated freeze/thaw cycles
General description
Fragment mixture prepared by cleavage of pBR322 DNA with restriction endonuclease Hae III.
Size Range: 8 to 587 bp
Size Range: 8 to 587 bp
Application
DNA Molecular Weight Marker V is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
<UL><LI>Restriction digests</LI>
<LI>PCR</LI>
<LI>RT-PCR</LI></UL>
<UL><LI>Restriction digests</LI>
<LI>PCR</LI>
<LI>RT-PCR</LI></UL>
Components
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0)
Sequence
The mixture contains 22 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 8, 11, 18, 21, 51, 57, 64, 80, 89, 104, 123, 124, 184, 192, 213, 234, 267, 434, 458, 504, 540, and 587 as determined by computer analysis of the pBR322 sequence.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Unit Definition
50 μg = 1A260 unit
Preparation Note
Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents
Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.
Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.
Storage and Stability
Store at -15–-25 °C. (until use)
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
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Julia Romero et al.
Nature protocols, 3(11), 1729-1735 (2008-10-18)
The lamin B2 locus is the only mammalian origin whose replication initiation points (RIPs) have been mapped. Although this paper was published 8 years ago, no further mammalian RIP-mapping studies have been reported, largely due to technical difficulties of ligation-mediated
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