10814270001
Roche
Primer for cDNA Synthesis
lyophilized, pkg of 40 μg
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Synonym(s):
oligo-p(dt)15
UNSPSC Code:
41106300
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form
lyophilized
Quality Level
packaging
pkg of 40 μg
manufacturer/tradename
Roche
storage temp.
−20°C (−15°C to −25°C)
Related Categories
General description
This primer consists of a 15-mer oligonucleotide that shows thymidine (T) at each individual position. The oligonucleotides are HPLC purified, desalted, and supplied as a lyophilizate of the Li-salt. The p(dT)15 primer is not phosphorylated at the 5′ end.Oligo(dT)N generates full-length cDNAs. If template contains oligo(A) stretches internally, the primer may bind these, causing mis priming and transcripts that are not full-length.
Composition: p(dT)15[ 15-mer oligonucleotide containing only deoxythymidine (dT) residues].
Phosphorylation: The p(dT)15 primer is not phosphorylated at the 5′ end.
Composition: p(dT)15[ 15-mer oligonucleotide containing only deoxythymidine (dT) residues].
Phosphorylation: The p(dT)15 primer is not phosphorylated at the 5′ end.
Application
Primer for cDNA synthesis may be used in:
- RT-PCR
- Generation of cDNA libraries
- Synthesis of first-strand cDNA
Unit Definition
8 nmol ξ 1 A260 unit
Other Notes
For life science research only. Not for use in diagnostic procedures.
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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