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Key Documents

10812846001

Roche

Tris hydrochloride

>99% (titration), pH 7.0-9.0, suitable for FISH

Synonym(s):

Trizma® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

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About This Item

Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein:
3675235
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:

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Assay

>99% (titration)

form

solid

packaging

pkg of 500 g

manufacturer/tradename

Roche

technique(s)

FISH: suitable

pH

7.0-9.0

useful pH range

7.0-9.0

pKa (25 °C)

8.1

absorption

<0.02 at 300 nm at 100 mg/mL

storage temp.

room temp

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This Item
6483139310OPT5941
technique(s)

FISH: suitable

technique(s)

-

technique(s)

-

technique(s)

DNA purification: suitable, cell culture | mammalian: suitable, electrophoresis: suitable, protein extraction: suitable

assay

>99% (titration)

assay

≥99% (titration)

assay

-

assay

≥99.0% (titration)

form

solid

form

solid

form

crystalline powder, needles

form

crystalline powder

storage temp.

room temp

storage temp.

15-25°C

storage temp.

-

storage temp.

-

pH

7.0-9.0

pH

-

pH

-

pH

-

packaging

pkg of 500 g

packaging

-

packaging

-

packaging

-

Application

Buffering agent in incubation mixtures.[1] It has also been used as a component of lysis and TE (Tris-EDTA) buffer.[2]

Features and Benefits

Crystals

Analysis Note

Purity: >99% Tris-HCl (titrimetric)
Heavy metals: <1 ppm Pb and Fe

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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