10812846001
Roche
Tris hydrochloride
>99% (titration), pH 7.0-9.0, suitable for FISH
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Synonym(s):
Trizma® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein:
3675235
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
Recommended Products
Assay
>99% (titration)
form
solid
packaging
pkg of 500 g
manufacturer/tradename
Roche
technique(s)
FISH: suitable
pH
7.0-9.0
useful pH range
7.0-9.0
pKa (25 °C)
8.1
absorption
<0.02 at 300 nm at 100 mg/mL
storage temp.
room temp
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
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Related Categories
Application
Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer.
Features and Benefits
Crystals
Quality
Purity: >99% Tris-HCl (titrimetric)
Heavy metals: <1 ppm Pb and Fe
Heavy metals: <1 ppm Pb and Fe
Other Notes
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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