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T3253

Sigma-Aldrich

Trizma® 盐酸盐 盐酸盐

reagent grade, ≥99.0% (titration), crystalline

别名:

TRIS 盐酸盐, 三羟基甲基氨基甲烷 盐酸盐, 三羟甲基氨基甲烷盐酸盐, 盐酸三甲胺

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About This Item

线性分子式:
NH2C(CH2OH)3 · HCl
CAS号:
1185-53-1
分子量:
157.60
Beilstein:
3675235
EC 号:
214-684-5
MDL编号:
MFCD00012590
UNSPSC代码:
12161700
PubChem化学物质编号:
24900119
NACRES:
NA.25

等级

reagent grade

质量水平

400

方案

≥99.0% (titration)

表单

crystalline

储存条件

dry at room temperature

技术

affinity chromatography: suitable

杂质

≤0.5% water (Karl Fischer)

颜色

white

有效pH范围

7.0-9.0

pKa (25 °C)

8.1

mp

150-152 °C

溶解性

H2O: soluble, clear, colorless

密度

1.39 g/cm3 at 20—25 °C
1.41 g/cm3 at 20—25 °C

吸附

≤0.05 at 290 nm at 40%

适用性

suitable for mass spectrometry

应用

diagnostic assay manufacturing
life science and biopharma
sample preparation

SMILES字符串

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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相关类别

一般描述

Tris Hcl，即三羟甲基氨基甲烷®盐酸盐，是一种广泛使用的酸性缓冲液，生理 pH 范围为 7.3 ~ 7.5。25°C时 pKa 为 8.08，pH 值范围为 7.0 至 9.0，与生物体的典型 pH 值一致。在各种生物学、细胞生物学和生物化学应用中，Tris Hcl 对于有效控制缓冲溶液得 pH 值至关重要。值得注意的是，单独的 Tris 碱和 Tris 盐酸盐缺乏足够的缓冲能力，促使它们在 pH 7 ~ 9 范围内结合。

Tris 缓冲液在蛋白质电泳和蛋白质印迹等技术中发挥着关键作用，可作为蛋白质转移的低离子强度缓冲液。它们也是SDS-PAGE凝胶，运行缓冲液和印迹缓冲液的组成部分。此外，Tris 盐，特别是在 TAE 和 TBE 缓冲液中，广泛用于 DNA 琼脂糖电泳。这些缓冲液在生物化学、蛋白质纯化和电泳等多种应用中发挥着关键作用，展示了 Tris HCl 在生物化学和细胞生物学研究中维持最佳 pH 值的通用性和重要性。

应用

Trizma®盐酸已作为缓冲溶液用于染色过程、还原和烷基化研究以及原位杂交过程。-

特点和优势

  • 非常适合用作细胞生物学和生化研究中的生物学缓冲液
  • 有效缓冲 pH 范围 7-9 (25 °C)，pKa为 8.1 (25 °C)
  • 经过测试，确认低水平的重金属污染，确保各种应用的适用性


其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
如需了解生化试剂系列的更多信息，请填写此表。
所有缓冲液的pH值都与温度和浓度有关。对于 Tris 缓冲液，温度每降低 1°C，pH 就会增加约 0.03 单位，每稀释 10 倍，pH 会降低 0.03-0.05 单位。对于一些精准的应用，请使用经过仔细校准的pH计和玻璃/甘汞组合电极。

法律信息

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000414299
0000414308
0000414309
0000414312
0000414315

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the pH of Trizma HCl, Product T3253, in solution?

    The pH of Trizma hydrochloride in water is dependent on the concentration. The pH of a 0.1 M aqueous solution is approximately 4.7 while the pH of a 40% (w/w) aqueous solution is typically 2.6-3.3.

  4. How can I find the technical bulletin 106B that is referenced in the Product Information Sheet for Product T3253, Trizma® hydrochloride?

    Under the Documents section above.

  5. Is Product T3253, Trizma® hydrochloride, the same as Tris buffer?

    No. Trizma HCl in solution at 0.05 M will produce a pH of approximately 4.7, but will have little if any buffering capacity. Trizma Base in solution at 0.05 M will produce a pH of approximately 10.4, but will have little if any buffering capacity. Blending Trizma Base and Trizma HCl will produce any desired pH between 7 and 9, with a reasonable buffering capacity.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Evaluation of the allergenicity and antigenicity of bovine-milk alphas1-casein using extensively purified synthetic peptides.
Elsayed S et al.
Scandinavian Journal of Immunology, 60, 486-486 (2004)
Differential effects of delayed aging on phenotype and striatal pathology in a murine model of Huntington disease.
Tallaksen-Greene SJ et al.
The Journal of Neuroscience, 34, 15658-15658 (2014)
Sandra Lawrynowicz Leibel et al.
Scientific reports, 9(1), 13450-13450 (2019-09-19)
Surfactant protein B (SFTPB) deficiency is a fatal disease affecting newborn infants. Surfactant is produced by alveolar type II cells which can be differentiated in vitro from patient specific induced pluripotent stem cell (iPSC)-derived lung organoids. Here we show the
Tris-tris-HCl: a standard buffer for use in the physiologic pH range.
R A Durst et al.
Clinical chemistry, 18(3), 206-208 (1972-03-01)
Marcel Kuiper et al.
Biotechnology progress, 35(4), e2821-e2821 (2019-04-16)
Perfusion is a cell culture mode that is gaining popularity for the manufacture of monoclonal antibodies and their derivatives. The cell culture media supporting perfusion culture need to support higher cell densities than those used in fed-batch culture. Therefore, when
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实验方案

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌制PAGE和SDS-PAGE凝胶

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

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