10354074001
Roche
Citrate Lyase (CL)
from Klebsiella pneumoniae (Aerobacter aerogenes)
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biological source
bacterial (Klebsiella pneumoniae)
Quality Level
form
lyophilized
specific activity
≥0.25 U/mg (At 25 °C with citrate as the substrate.)
packaging
pkg of 120 U
manufacturer/tradename
Roche
technique(s)
activity assay: suitable
color
light cream
optimum pH
8.0-9.0
solubility
water: soluble
NCBI accession no.
UniProt accession no.
application(s)
life science and biopharma
foreign activity
ICDH <0.05% (NAD as coenzyme)
NADH oxidase <0.05%
storage temp.
2-8°C
Related Categories
General description
Citrate lyase is considered as a substrate- induced enzyme which helps in catalyzing reversible aldol cleavage of citrate to oxaloacetate and acetate. The enzyme is adaptive to anaerobic dissimilation of citrate.
Application
It was used in enzymatic assay to measure citrate concentration using microtiter plates.
Quality
Contaminants: <0.05% ICDH (NAD specific) and “NADH oxidase”, each
Physical form
Lyophilizate, stabilized with BSA, sucrose, MgSO4 and EDTA. Note: A solution in double-distilled water has a pH-value of approximately 7.0. The enzyme can be relyophilized.
Preparation Note
Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+)
Other Notes
For life science research only. Not for use in diagnostic procedures.
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
含少量动物源组分生物产品
常规特殊物品
Certificates of Analysis (COA)
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Agata M Pudlik et al.
Journal of bacteriology, 193(3), 706-714 (2010-12-01)
Carbohydrate/citrate cometabolism in Lactococcus lactis results in the formation of the flavor compound acetoin. Resting cells of strain IL1403(pFL3) rapidly consumed citrate while producing acetoin when substoichiometric concentrations of glucose or l-lactate were present. A proton motive force was generated
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