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10295892001

Roche

Colcemid

solution, suitable for blocking, sterile; 0.2 μm filtered

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Synonym(s):
Demecolcine, N-Deacetyl-N-methylcolchicine
Empirical Formula (Hill Notation):
C21H25NO5
CAS Number:
477-30-5
Molecular Weight:
371.43
Beilstein:
2822892
MDL number:
MFCD00075459
UNSPSC Code:
12352207
PubChem Substance ID:
329749009

description

N-methyl-N-deacetyl-colchicine

Quality Level

100

sterility

sterile; 0.2 μm filtered

form

solution

packaging

pkg of 20 mL (10 μg/ml)

manufacturer/tradename

Roche

technique(s)

blocking: suitable

impurities

microbial, tested

solubility

water: miscible

storage temp.

2-8°C

SMILES string

CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13

InChI

1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1

InChI key

NNJPGOLRFBJNIW-HNNXBMFYSA-N

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General description

Colcemid is also known as demecolcine. Its generic name is N-methyl-N-deacetyl-colchicine. Colcemid depolymerizes microtubules and blocks mitosis at metaphase.

Application

Colcemid inhibits the formation of mitotic spindles. It is used to increase the percentage of metaphase cells for chromosome analysis.

Biochem/physiol Actions

Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.

Physical form

Solution (10 μg/ml), filtered through 0.2 μm pore size membrane.

Other Notes

For life science research only. Not for use in diagnostic procedures.

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cloning and stem cells, 11(1), 141-152 (2009-02-20)
This study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to
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Chromosomal instability (CIN) is a hallmark of many cancers. Restricting the localization of centromeric histone H3 variant CENP-A to centromeres prevents CIN. CENP-A overexpression (OE) and mislocalization have been observed in cancers and correlate with poor prognosis; however, the molecular
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