10280879001
Roche
5-Bromo-2′-deoxyuridine
>97%, crystalline solid, pkg of 1 g
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Synonym(s):
5-Bromo-2′-deoxyuridine, brdu, 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
Empirical Formula (Hill Notation):
C9H11BrN2O5
CAS Number:
Molecular Weight:
307.10
Beilstein:
30395
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
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Quality Level
Assay
>97%
form
crystalline solid
mol wt
307.1
packaging
pkg of 1 g
manufacturer/tradename
Roche
mp
191-194 °C (dec.) (lit.)
shipped in
wet ice
storage temp.
2-8°C
SMILES string
OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
InChI
1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
InChI key
WOVKYSAHUYNSMH-RRKCRQDMSA-N
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General description
It is conventionally used as a marker for S-phase synthesis of DNA.
Application
5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies.
Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.
Biochem/physiol Actions
Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.
Quality
Purity: 97% (from N), chromatographically homogeneous
Preparation Note
Working concentration: According to literature for cell culture, a final concentration of 10 μmol/l has been used.
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.
Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.
Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C
Other Notes
For life science research only. Not for use in diagnostic procedures.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Muta. 1B - Repr. 2
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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American journal of physiology. Cell physiology, 285(2), C253-C259 (2003-04-04)
The present study was designed to develop methods to study the production and release of monocytes from the bone marrow using the thymidine analog 5'-bromo-2'-deoxyuridine (BrdU). Dividing monocytes in bone marrow were labeled with BrdU (MOBrdU), and their release into
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The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 41(1), 1-6 (1993-01-01)
Proliferating cell nuclear antigen (PCNA) was evaluated as a marker of cell proliferation in formalin-fixed rat liver tissue through a comparative study with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU). The comparison was conducted through the introduction of a dual immunohistochemical procedure
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