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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
Product Name
GTP-γ-S, Tetralithium salt
color
white
description
C10H12N5O13P3SLi4
assay
87% (HPLC)
form
solid
mol wt
Mr 539.2 (GTP-γ-S)
Mr 563.0 (GTP-γ-S-Li4)
composition
GDP, ≤12% HPLC
GMP, ≤1% HPLC
GTP, ≤2% HPLC
packaging
pkg of 10 mg
manufacturer/tradename
Roche
storage condition
protect from light
concentration
97%
technique(s)
activity assay: suitable
solubility
water: soluble
storage temp.
−20°C (−15°C to −25°C)
Quality Level
Related Categories
Analysis Note
Absorption: Content is measured in aqueous solution by absorption at 254 nm.
Conditions:
Conditions:
- approx. 10 mg/ml double-dist. water
- dilution: 9.8 ml double-dist. water + 0.2 ml sample.
Application
GTP-γ-S has been used:
- in G-Protein activation assay to assess the functionality of protease-activated receptor 4 (PAR4)
- in fluorimetric guanine nucleotide exchange assay of G protein alpha subunits (Gα)
- to monitor the Rac family small guanosine triphosphatase (GTPase) 2 (Rac2)-stimulated activity of phospholipase Cγ2 mutants
Biochem/physiol Actions
Guanosine 5′-O-(3-thiotriphosphate) (GTP-γ-S) activates guanine-nucleotide-binding proteins and is slowly hydrolyzed enzymatically. It inhibits guanosine triphosphatase (GTPases) more potently than guanosine triphosphate (GTP),
General description
Guanosine 5′-O-(3-thiotriphosphate), tetralithium salt (GTP-γ-S) is a non-hydrolysable analog of GTP. It is an inhibitor of phosphodiesterase in photoreceptors.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Preparation Note
Store powder dry at -15 to -25 °C; aqueous solutions with pH approx. 7 should be stable for six months at -15 to -25 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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Claudia Walliser et al.
The Journal of biological chemistry, 291(42), 22136-22148 (2016-08-21)
Mutations in the gene encoding phospholipase C-γ2 (PLCγ2) have been shown to be associated with resistance to targeted therapy of chronic lymphocytic leukemia (CLL) with the Bruton's tyrosine kinase inhibitor ibrutinib. The fact that two of these mutations, R665W and
S Nawy et al.
Neuron, 7(4), 677-683 (1991-10-01)
Transmitter release from photoreceptors is decreased by light, resulting in a conductance increase in depolarizing bipolar cells. Addition of exogenous cGMP through a patch pipette to depolarizing bipolar cells from slices of dark-adapted tiger salamander retina resulted in an enhancement
Stephen R Sprang
Biopolymers, 105(8), 449-462 (2016-03-22)
This review addresses the regulatory consequences of the binding of GTP to the alpha subunits (Gα) of heterotrimeric G proteins, the reaction mechanism of GTP hydrolysis catalyzed by Gα and the means by which GTPase activating proteins (GAPs) stimulate the
Gilbert Di Paolo, Markus R. Wenk
Lipids, 3-18 (2012)
K Sasaki et al.
Nature, 325(6101), 259-262 (1987-01-15)
Recently, a GTP-binding protein sensitive to islet activating protein (IAP) has been suggested to be important in producing K+-currents when the muscarinic receptor of the atrial muscle is activated by acetylcholine (ACh). Here we confirm the blocking effects of IAP
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