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biological source
bacteriophage MS2
Quality Level
form
solution
mol wt
~1200 kDa
packaging
pkg of 500 μL (10 A260 units)
manufacturer/tradename
Roche
technique(s)
RNA extraction: suitable
color
colorless
solubility
water: miscible
storage temp.
−20°C (−15°C to −25°C)
Related Categories
General description
RNA, from bacteriophage MS2 is composed of 3569 nucleotides. MS2 bacteriophage acts as a model system in studying molecular biology processes, such as viral RNA replication, process of translation and physiology of infected cells. MS2 RNA codes for three viral polypeptides namely, protein A, coat protein and RNA replicase complex. Protein A and coat protein make up the structure of MS2 virus.
Application
RNA, MS2 has been used:
- as a standard to assess RNA quantification methods
- in RNA extraction
- in single-tube, fluorescent PERT assay (STF-PERT) for accurate quantitation of different retrovirus types
- for studies which use natural RNA in a in vivo and in vitro protein-synthesizing system, initiation, elongation, and termination of polypeptide synthesis.
- for structural and functional studies.
- to stabilize RNA during cDNA synthesis
- to improve RNA yield during RNA extraction or isolation
Quality
Typical analysis: Free of protein and host nucleic acids according to current Quality Control procedures.
Sequence
Chain Length 3,569 nucleotides
Preparation Note
Working solution: Storage buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 7.0.
Other Notes
For life science research only. Not for use in diagnostic procedures.
WGK
nwg
Flash Point(F)
No data available
Flash Point(C)
No data available
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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