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biological source
papaya
Quality Level
form
suspension
specific activity
30 U/mg
~30 units/mg protein (At 25 °C with BAEE as the substrate.)
mol wt
23 kDa
packaging
pkg of 10 mL (100 mg)
manufacturer/tradename
Roche
concentration
10-100 mg/mL
optimum pH
6.0-7.0
shipped in
wet ice
Related Categories
General description
Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.
Application
Papain has been used in the digestion of various brain cells.
Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.
Biochem/physiol Actions
Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.
Features and Benefits
Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals
Physical form
Suspension, crystalline, nonsterile
Preparation Note
Working concentration: 0.05 to 0.5 mg/ml
Storage and Stability
Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)
Other Notes
For life science research only. Not for use in diagnostic procedures.
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
动植物源性产品
Certificates of Analysis (COA)
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The structure of papain
Advances in Protein Chemistry, 25, 79-115 (1971)
Logan A Walker et al.
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