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Roche

DIG Oligonucleotide Tailing Kit, 2nd generation

greener alternative

sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide)

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About This Item

NACRES:
NA.55
UNSPSC Code:
41105500

Key Documents

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Product Name

DIG Oligonucleotide Tailing Kit, 2nd generation, sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide)

usage

sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide)

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

Aligned,

Quality Level

100

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Application

DIG Oligonucleotide Tailing Kit, 2nd generation has been used to label oligonucleotide probes in:
  • northern blot assay
  • in situ hybridization (ISH)
  • fluorescence in situ hybridization (FISH)
In addition to common hybridization techniques, DIG-labeled oligonucleotides are especially useful for screening expression libraries for sequence-specific DNA binding proteins, for example, transcription factors.

Biochem/physiol Actions

DIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide(s).

Features and Benefits

Tailing of oligonucleotides at the 3′-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10 – 100).

  • Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides
  • Fast hybridization kinetics, due to the small size of oligonucleotides
  • Single-stranded probes, no renaturation during hybridization
  • Sequence can be designed according to the experiment
  • Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells

General description

DIG Oligonucleotide Tailing Kit, 2nd generation employs the enzyme terminal transferase. It catalyzes the addition of digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) and deoxyadenosine triphosphate (dATP) to the 3′-OH end of oligonucleotides.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 kit containing 11 components

Preparation Note

Working concentration: Oligonucleotides
In one standard labeling reaction up to 100 pmol oligonucleotide (1 μg of a 30-mer oligonucleotide) can be applied.
Store at -15–-25 °C. (unopened kit)

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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B Inhalation - Repr. 1B

Storage Class

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash

Regulatory Information

常规特殊物品
含少量动物源组分生物产品
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Certificates of Analysis (COA)

Lot/Batch Number
46518320
38583420
20812921
14118021
10623721

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Xiaoming Li et al.
Nucleic acids research, 32(3), 867-877 (2004-02-13)
We report here the biochemical characterization of the deafness-associated mitochondrial tRNA(Ser(UCN)) T7511C mutation, in conjunction with homoplasmic ND1 T3308C and tRNA(Ala) T5655C mutations using cybrids constructed by transferring mitochondria from lymphoblastoid cell lines derived from an African family into human
Amanda Dixon-McIver et al.
PloS one, 3(5), e2141-e2141 (2008-05-15)
Acute myeloid leukaemia (AML) is the most common acute leukaemia in adults; however, the genetic aetiology of the disease is not yet fully understood. A quantitative expression profile analysis of 157 mature miRNAs was performed on 100 AML patients representing
A small regulatory RNA contributes to the preferential colonization of Escherichia coli O157: H7 in the large intestine in response to a low DNA concentration
<BIG>Han R, et al.</BIG>
Frontiers in Microbiology, 8, 274-274 (2017)
Kaposi's sarcoma-associated herpesvirus mRNA accumulation in nuclear foci is influenced by viral DNA replication and viral noncoding polyadenylated nuclear RNA
<BIG>Vallery TK, et al.</BIG>
Journal of Virology, 92, e00220-e00218 (2018)
L1CAM in the early enteric and urogenital system
<BIG>Pechriggl E, et al.</BIG>
The Journal of Histochemistry and Cytochemistry, 65, 21-32 (2017)
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Articles

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

地高辛 (DIG) 标记方法

地高辛（DIG）标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。

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