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Roche
Quick Spin High Capacity Columns
pkg of 20 columns
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General description
Quick Spin High Capacity Columns are produced with material shown to have recovery of DNA (≥90%). All the tedious, time-consuming steps involved in preparing columns have already been performed. The columns are pre-swollen, pre-packed, pre-spun, and quality tested to ensure
- maximal retention of unincorporated nucleotides (≥98%), and
- absence of DNase contaminaton according to the current Quality Control procedures.
Application
Quick Spin High Capacity G-50 Sephadex columns has been used to desalt viral samples. It has been used during the purification process in order to remove humic substances from RNA samples.
Features and Benefits
Exclusion limit for ds DNA: >72 base pairs
Recovery: =90% of DNA
Retention: =98% of unincorporated nucleotides
Sample size: 100 to 500 μl
Recovery: =90% of DNA
Retention: =98% of unincorporated nucleotides
Sample size: 100 to 500 μl
Physical form
Pre-packed, pre-swollen, columns (3.0 ml bed volume). Suspension of Sephadex G-50 in TE buffer (10 mM Tris, pH 8.0; 1 mM EDTA).
Storage and Stability
Store at 2 to 8 °C. (Do not freeze. To avoid contamination of the columns and collecting tubes, leave them in the storage bag until just before use.)
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
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