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Merck
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Y04C-02-5PK

CellASIC ONIX plate for haploid yeast cells (4 chamber, 3.5-5 micron)

The Y04 plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining x,y position over time.

Synonym(s):

Haploid Yeast Cell Plate, Haploid Yeast Plate, ONIX Yeast Plate

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About This Item

UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32011202
Material:
PMMA , glass , polycarbonate , polydimethylsiloxane (PDMS)
Manufacturer/tradename:
CellASIC® ONIX
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material

PMMA , glass , polycarbonate , polydimethylsiloxane (PDMS)

manufacturer/tradename

CellASIC® ONIX

W × L × H

85.48 mm × 127.76 mm × 14.35 mm

compatibility

for use with cells sized 3.5–5.0 μm

shipped in

ambient

General description

The Y04 plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining the x,y position over time.

Application

CellASIC® ONIX plate for haploid yeast cells (4chamber, 3.5-5 micron) has been used in live-cell microscopy and analysis of Schizosaccharomyces pombe cells. It has also been used in live cell imagining of Saccharomyces cerevisiae strains. This CellASIC ONIX plate for haploid yeast cells (4chamber, 3.5-5 micron) is also suitable for:
  • Time-lapse analysis of yeast cells
  • Long-term continuous perfusion experiments
  • Solution exchange experiments (induction, inhibition, drug dosing, etc.)
  • Comparison of up to 4 different cell types or exposure conditions(media components) in parallel
  • Cell division tracking (follow mother/daughter cells over generations)
  • Temperature and gas atmospheric control (temperature shift, anoxic conditions etc.)

Features and Benefits

  • Compatible with CellASIC® ONIX2 Microfluidic system and ONIX2 Manifolds
  • Enables perfusion-based, long-term, live-cell analysis with solutionswitching
  • The plate offers a controlled and dynamicmicroenvironment for cells

Legal Information

CELLASIC is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chia-Wei Lee et al.
Nature cell biology, 22(2), 159-166 (2020-02-08)
Nuclear pore complexes (NPCs) are very large proteinaceous assemblies that consist of more than 500 individual proteins1,2. NPCs are essential for nucleocytoplasmic transport of different cellular components, and disruption of the integrity of NPCs has been linked to aging, cancer
Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors.
Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB
The Biochemical Journal, 432(2), 399-406 (2010)
Oscillations in CDC14 release and sequestration reveal a circuit underlying mitotic exit.
Romilde Manzoni, Francesca Montani, Clara Visintin, Fabrice Caudron, Andres Ciliberto et al.
The Journal of cell biology, vol. 190 no. 2, 209-222 (2010)
Cytosolic pH is a second messenger for glucose and regulates the PKA pathway through V-ATPase.
Reinhard Dechant, Matteo Binda, Sung Sik Lee, Serge Pelet, Joris Winderickx and Matthias Peter
The Embo Journal, 29, 2515-2526 (2010)
Spinning-disk confocal microscopy of yeast.
Kurt Thorn
Test, Volume 470, 581-602 (2010)

Related Content

Convert your microscope to a live cell imaging system – the CellASIC® ONIX2 system allows precise manipulation of the cell culture environment for dynamic live cell imaging and analysis using mammalian, yeast, and bacterial cells.

We used the CellASIC™ ONIX MicroFluidic System, in conjunction with the M04S microfluidic plate, to achieve perfusion-based microenvironment control and study drug-induced cell death of 3D cultures of MCF7 cells in Matrigel®. The microfluidic chamber recreates the physiologic mass transport condition for optimized cell health. Four upstream fluidic channels allow controlled exposure of the cells to different solutions. The plate can also be cultured in a standard incubator using a dedicated gravity-driven flow channel. The cells are in contact with a #1.5 thickness (170 μm) optical glass surface, enabling high quality imaging using an inverted microscope. An integrated micro-incubator system delivers temperature and gas control to the microfluidic chambers.

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