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Merck
CN

WBULS0100

Immobilon ECL Ultra Western HRP Substrate

Western blotting, HRP

Synonym(s):

HRP substrate

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About This Item

UNSPSC Code:
41116010
NACRES:
NB.22
eCl@ss:
42029053

Key Documents

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Product Name

Immobilon ECL Ultra Western HRP Substrate, Western blotting, HRP

technique(s)

western blot: suitable

detection method

chemiluminescent

shipped in

ambient

Quality Level

300

Related Categories

Application

Immobilon® ECL Ultra substrate is compatible with both PVDF andnitrocellulose blotting membranes, as well as commonly used buffers and blocking reagents.

Sufficient for 1000 cm²

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immobilon® ECL Ultra Western HRP Substrate is an ultra-sensitive reagent with long signal duration for the detection of horseradish peroxidase (HRP)-labeled antibodies on western blots. The intense signal created permits sensitive detection at the mid-to-low femtogram range using x-ray film or digital imaging systems.

Other Notes

Solution A- 50 mL

Solution B- 50 mL

Preparation Note

Store at 2–8 °C

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Immunodetection

The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protocols

Introduction to Western Blot Detection of Proteins

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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