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WBAVDP001

Millipore

Immobilon® Block - PO (Phosphoprotein Blocker)

Western blotting, dot/slot blotting, mass spectrometry, phosphorolation assay

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UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22

Quality Level

packaging

pkg of 500 mL

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

membrane area

1000 cm2 , membrane coverage

detection method

chemiluminescent (phosphoprotein)
fluorometric (phosphoprotein)

shipped in

ambient

storage temp.

15-25°C

Quality

For optimum results, use Immobilon-P membrane for chemiluminescence (luminol) or visual detection (e.g. TMB), and Immobilon-FL membrane for fluorescent detection applications.

Storage and Stability

Stable for 1 year at 20–25°C

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H N Gao et al.
Journal of dairy science, 100(9), 7696-7709 (2017-06-26)
The ratio of different AA in the diets of cows is vital to improve milk protein yield. β-Casein is one of the important milk proteins with high nutritive value. However, the suitable ratio of essential amino acids (EAA) for the
Vanesa D Ramseyer et al.
American journal of physiology. Renal physiology, 308(2), F149-F156 (2014-11-08)
Thick ascending limbs reabsorb 30% of the filtered NaCl load. Nitric oxide (NO) produced by NO synthase 3 (NOS3) inhibits NaCl transport by this segment. In contrast, chronic angiotensin II (ANG II) infusion increases net thick ascending limb transport. NOS3
I-Ting Chen et al.
EMBO reports, 22(12), e52254-e52254 (2021-10-12)
Promyelocytic leukemia protein (PML) is a tumor suppressor possessing multiple modes of action, including induction of apoptosis. We unexpectedly find that PML promotes necroptosis in addition to apoptosis, with Pml-/- macrophages being more resistant to TNF-mediated necroptosis than wild-type counterparts
Hai-na Gao et al.
Journal of Zhejiang University. Science. B, 16(6), 560-572 (2015-06-10)
The aim of this study is to investigate the effects of leucine (Leu) and histidine (His) on the expression of both the mammalian target of rapamycin (mTOR) signaling pathway-related proteins and caseins in immortalized bovine mammary epithelial cells (CMEC-H), using

Articles

The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots

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