Skip to Content
Merck
CN

WBAVDFL01

Immobilon® Block - FL (Fluorescent Blocker)

Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays

Synonym(s):

Fluorescent Blocker, Immobilon Blocking Reagent

Sign In to View Organizational & Contract Pricing.

Select a Size


About This Item

UNSPSC Code:
41116010
NACRES:
NB.22
eCl@ss:
42029053
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Immobilon® Block - FL (Fluorescent Blocker), Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays

packaging

pkg of 500 mL

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

membrane area

1000 cm2 , membrane coverage

detection method

fluorometric

shipped in

ambient

storage temp.

15-25°C

Quality Level

Analysis Note

For optimum results, use Immobilon-P membrane for chemiluminescence (luminol) or visual detection (e.g. TMB), and Immobilon-FL membrane for fluorescent detection applications.

Application

For use on immunoblots.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immobilon® Block Noise Cancelling Reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimized to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection. Bløk buffers are ideal for use with Immobilon-P Western blotting membranes and the SNAP i.d. system, and are also compatible with standard Western blotting techniques.

Packaging

Sufficient reagent for 19 mini blots (8x7 cm)

Preparation Note

Stable for 2 years at 20–25°C

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation markEnvironment

signalword

Warning

flash_point_f

does not flash

flash_point_c

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Yann Ehinger et al.
EMBO molecular medicine, 12(2), e10889-e10889 (2020-01-09)
Mutations in the X-linked MECP2 gene are responsible for Rett syndrome (RTT), a severe neurological disorder for which there is no treatment. Several studies have linked the loss of MeCP2 function to alterations of brain-derived neurotrophic factor (BDNF) levels, but

Articles

荧光Western blot检测因具有多重检测的方便性而变得越来越受研究者的欢迎,但这种检测仍需要专门优化的工具来获得理想的实验结果。这里我们推荐一个最佳组合,以帮助大家在荧光检测中快速获得稳定、易重复的数据。

Related Content

我们明白。西部片的污点可能会让人头疼,我们当然也有过这样的经历。因此,请与我们分享您的丑陋污点,并获得一份惊喜!虽然我们不能告诉你是什么--那会破坏惊喜的气氛--但它会激发实验室的创造力。

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service