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UFC30DV0S

Millipore

Ultrafree® Centrifugal Filter, 0.5 mL Sample Volume

pore size 0.65 μm, PVDF membrane (hydrophilic), sterile

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Synonym(s):
Centrifuge filter
UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24

material

PVDF membrane (hydrophilic)
polypropylene

Quality Level

400

sterility

sterile

feature

holdup volume 5 μL

manufacturer/tradename

Ultrafree®

parameter

0.5 mL sample volume
50 °C max. temp.

technique(s)

protein extraction: suitable
protein purification: suitable

L

45 mm

diam.

10.6 mm

filtration area

0.2 cm2

matrix

Durapore®

pore size

0.65 μm pore size

application(s)

sample preparation

shipped in

ambient

Related Categories

General description

Filter Code: DVPP
Filter Type: Microfiltration
Ultrafree®-MC is a single-use, disposable filter used for removing particulates from aqueous biological solutions.

Application

Ultrafree®-MC Centrifugal Filter has been used:
  • to filter extracellular vesicle (EV) from seminal plasma
  • in the purification of single-strand-selective uracil-DNA glycosylase (SMUG1)
  • to the purification of EVs for nanoparticle tracking analysis

Physical form

Color Code: Purple

Legal Information

Durapore is a registered trademark of Merck KGaA, Darmstadt, Germany
Ultrafree is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

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Janis A Müller et al.
Nature communications, 9(1), 2207-2207 (2018-06-09)
Zika virus (ZIKV) causes severe birth defects and can be transmitted via sexual intercourse. Semen from ZIKV-infected individuals contains high viral loads and may therefore serve as an important vector for virus transmission. Here we analyze the effect of semen
Ruth E Thomas et al.
PLoS genetics, 14(9), e1007694-e1007694 (2018-09-27)
Mutations in the glucosylceramidase beta (GBA) gene are strongly associated with neurodegenerative diseases marked by protein aggregation. GBA encodes the lysosomal enzyme glucocerebrosidase, which breaks down glucosylceramide. A common explanation for the link between GBA mutations and protein aggregation is
Penelope Kroustallaki et al.
Cell reports, 28(7), 1690-1702 (2019-08-15)
Telomerase biogenesis is a complex process where several steps remain poorly understood. Single-strand-selective uracil-DNA glycosylase (SMUG1) associates with the DKC1-containing H/ACA ribonucleoprotein complex, which is essential for telomerase biogenesis. Herein, we show that SMUG1 interacts with the telomeric RNA component

Protocols

Affinity Purification with Ultrafree®-MC Centrifugal Filters

Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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