Skip to Content
Merck
CN

UFC201024

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

greener alternative

sample volume 2 mL, regenerated cellulose membrane, MWCO 10 kDa

Synonym(s):

Centrifugal concentrator, 10 kDa MWCO, 2 mL sample volume

Sign In to View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

material

polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing

Quality Level

400

sterility

non-sterile

product line

Amicon®

packaging

pkg of 24 pkg

manufacturer/tradename

Amicon® Ultra

sustainability

Greener Alternative Product

parameter

2 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

L

119.7 mm

diam.

15.9 mm

filtration area

1 cm2

volume

2 mL

working volume

≤2 mL
2 mL

pore size

10 kDa MWCO

greener alternative category

Re-engineered

shipped in

ambient

Related Categories

General description

Amicon® Ultra-2 centrifugal filter devices offer fast ultrafiltration. It has the potential to recover high concentration factors and easy concentrate from dilute and complex sample matrices. Amicon® Ultra-2 devices are supplied non-sterile and are for single use only.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

Application

  • Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates.
  • Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
  • Desalting, buffer exchange and protein dialysis.
  • Size exclusion and protein fractionation.
Amicon® Ultra-2 Centrifugal Filter Unit has been used:
  • to reduce large volume biofluids prior to extracellular vesicle (EV) isolation or to concentrate EVs
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids (DNA/RNA samples, either single- or double-stranded), microorganisms, column eluates, and purified samples
  • Purification of macromolecular components found in tissue culture extracts and cell lysates, removal of primer, linkers, or molecular labels from a reaction mix, and protein removal before high-performance liquid chromatography (HPLC)
  • Desalting, buffer exchange, or diafiltration

Features and Benefits

  • Amicon® Ultra 2ml 10K 24PK
  • Concentrates 2 mL down to 15-70 μl
  • 98% sample recovery
  • Fast processing time - 10 to 30 minute spin times
  • Engineered dead stop
  • The reverse spin capability provides consistent recoveries

  • Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
  • Concentrates 2 mL down to 15-70 μL
  • 98% sample recovery
  • Fast processing time: 10 to 30-minute spin times
  • Engineered dead stop
  • Reverse spin capability provides consistent recoveries

Other Notes

Replaces: 4205
The Amicon® Ultra-2 device is supplied with two tubes. During operation, one tube is used to collect filtrate; the other to cap the device during concentration and subsequently to recover the concentrated sample. Amicon Ultra-2 Centrifugal filter devices are for research use only and not for use in diagnostic procedures.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Bao Quoc Tran et al.
Data in brief, 6, 68-76 (2016-01-23)
A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated
Lulai Xu et al.
Vaccine, 38(7), 1690-1699 (2020-01-16)
Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene delivery. In the present study, we generated the thermostable recombinant NDV (rNDV) expressing the different forms of hemagglutinin (HA) of highly pathogenic avian
Keerthie Dissanayake et al.
Theriogenology, 149, 104-116 (2020-04-08)
Extracellular vesicles (EVs) are membrane-bound biological nanoparticles (NPs) and have gained wide attention as potential biomarkers. We aimed to isolate and characterize EVs from media conditioned by individually cultured preimplantation bovine embryos and to assess their relationship with embryo quality.
Glenn Vergauwen et al.
Scientific reports, 7(1), 2704-2704 (2017-06-04)
Identification and validation of extracellular vesicle (EV)-associated biomarkers requires robust isolation and characterization protocols. We assessed the impact of some commonly implemented pre-analytical, analytical and post-analytical variables in EV research. Centrifugal filters with different membrane types and pore sizes are
Jasjeet Kaur et al.
Virus genes, 56(4), 480-497 (2020-05-06)
Staphylococcus aureus is one of the most dreadful infectious agents, responsible for high mortality and morbidity in both humans and animals. The increased prevalence of multidrug-resistant (MDR) Staphylococcus aureus strains has limited the number of available treatment options, which calls

Articles

Protein Sample Ultrafiltration

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

蛋白样品超滤

本页内容讨论了使用超滤膜进行蛋白样品制备的原理和优势。应用包括溶剂置换、蛋白样品浓缩或纯化。

Protocols

Extracellular Vesicle Protocol

Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.

Related Content

Amicon® Ultra离心超滤管

浓缩你的蛋白质，别失去它。使用Amicon® Ultra过滤器进行浓缩，能可靠回收蛋白质。

Amicon Ultra遠心式限外ろ過フィルターユニット
メルク分析関連製品ガイド2017
Amicon®Ultra 2 mL Centrifugal Filters for Protein Purification and Concentration

"The Amicon® Ultra 2 mL filter provides a fast and easy way to concentrate, desalt and exchange thebuffer for both proteins and nucleic acids. Now you can concentrate 2 mL down to 15 – 70 μL in anAmicon® Ultra centrifugal device."

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service