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Key Documents

TMS-008

Sigma-Aldrich

Alizarin-Red Staining Solution

Alizarin red is a commonly used stain to identify calcium containing osteocytes in differentiated culture of both human and rodent mesenchymal stem cells (MSCs).

Synonym(s):

Alizarin Red S, Alizarin Red S Stain, Alizarin Red Stain

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500 ML
CN¥3,083.67

CN¥3,083.67


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500 ML
CN¥3,083.67

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

CN¥3,083.67


Please contact Customer Service for Availability

Quality Level

concentration

40 mM in distilled water

technique(s)

cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable

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This Item
SCM083SCM005SCM004
form

liquid

form

liquid

form

liquid

form

liquid

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Quality Level

300

Quality Level

300

Quality Level

100

Quality Level

100

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

input

sample type neural stem cell(s)

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)

input

sample type neural stem cell(s)

input

sample type neural stem cell(s)

General description

Alizarin Red, an anthraquinone derivative, may be used to identify calcium in tissue sections and cultured cells in vitro. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Calcium forms an Alizarin Red S-calcium complex in a chelation process, and the end product is a bright red stain. Alizarin red is a commonly used stain to identify calcium containing osteocyte in differentiated culture of both human and rodent mesenchymal stem cells.

Application

Research Category
Stem Cell Research
Research Sub Category
Osteobiology

Arthritis

Quality

Solution is red with no particulates present. Solution is suitable for endpoint cell culture assays.

Physical form

Product is presented as a 40mM solution at pH 4.1 +/- 0.1.

Storage and Stability

Store at 2-8ºC for up to 4 months from date of receipt. If Alizarin-red solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Articles

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Protocols

Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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