ST1630
Anti-FGF8 Mouse mAb (2A10)
liquid, clone 2A10, Calbiochem®
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Anti-Fibroblast Growth Factor 8
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biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
2A10, monoclonal
form
liquid
does not contain
preservative
species reactivity
mouse, human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Fgf8(14179)
General description
Anti-FGF8, mouse monoclonal, clone 2A10, recognizes the ~30-35 kDa FGF8 protein in PC-12, Jurkat, HepG2, NIH3T3, and Raw 264.7 cells. It is validated for use in ELISA and Western blotting.
Purified mouse monoclonal antibody. Recognizes the ~30-35 kDa FGF8 protein.
Recognizes the ~30-35 kDa FGF8 protein in PC-12, Jurkat, HepG2, NIH3T3, and Raw 264.7 cells.
Immunogen
Full-length, recombinant, human FGF8 (aa 65-133), expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Warning
Toxicity: Regulatory Review (Z)
Physical form
In PBS, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
PC-12 cells, Jurkat cells, HepG2 cells, NIH3T3 cells, Raw 264.7 cells
PC-12 cells, Jurkat cells, HepG2 cells, NIH3T3 cells, Raw 264.7 cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
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Certificates of Analysis (COA)
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