ST1568
Anti-DEFB4 Mouse mAb (4C4)
liquid, clone 4C4, Calbiochem®
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Synonym(s):
Anti-Defensin β4
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
4C4, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DEFB4A(1673)
General description
Purified mouse monoclonal antibody. Recognizes the DEFB4 protein.
Recognizes the DEFB4 protein.
This Anti-DEFB4 Mouse mAb (4C4) is validated for use in ELISA, Immunoblotting for the detection of DEFB4.
Immunogen
A recombinant polypeptide corresponding to amino acids 24-65 of human DEFB4, expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Warning
Toxicity: Regulatory Review (Z)
Physical form
In PBS, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Olivia McGinn et al.
Oncogene, 39(12), 2478-2492 (2020-01-29)
Estrogen receptor (ER) positive breast cancers often contain subpopulations of cells that express the intermediate filament protein cytokeratin 5 (CK5). CK5+ cells are enriched in cancer stem cell (CSC) properties, can be induced by progestins, and predict poor prognosis in
Jessica Finlay-Schultz et al.
Breast cancer research : BCR, 22(1), 68-68 (2020-06-25)
Breast cancer is a highly heterogeneous disease characterized by multiple histologic and molecular subtypes. While a myriad of breast cancer cell lines have been developed over the past 60 years, estrogen receptor alpha (ER)+ disease and some mutations associated with this
L M Fettig et al.
Oncogene, 36(44), 6074-6084 (2017-07-12)
Half of estrogen receptor-positive breast cancers contain a subpopulation of cytokeratin 5 (CK5)-expressing cells that are therapy resistant and exhibit increased cancer stem cell (CSC) properties. We and others have demonstrated that progesterone (P4) increases CK5+ breast cancer cells. We
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