All Photos(1)
Synonym(s):
Anti-T-Cell Lymphoma Invasion and Metastasis 1
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... TIAM1(7074)
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~200 kDa Tiam1 protein.
Recognizes the ~200 kDa Tiam1 protein in Jurkat and NIH3T3 cells.
This Anti-Tiam1 Rabbit pAb is validated for use in Immunoblotting for the detection of Tiam1.
Tiam1 (T-cell lymphoma invasion and metastasis 1) was originally identified by its ability to induce invasion by T-lymphoma cells when amplified. Tiam1 is a specific guanine nucleotide exchange factor (GEF) for the GTPase, Rac. By activating Rac1, Tiam1 regulates many cellular functions including actin cytoskeleton dynamics, cell proliferation, and cell survival. Tiam1 activity is regulated by its translocation to the membrane and the subsequent binding of its PH domain to phosphatidylinositol-3,4,5-triphosphate. Tiam1 is cleaved in a caspase-dependent manner in apoptotic cells, rendering it inactive as a result of an inability to translocate to the plasma membrane.
Immunogen
Human
a synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1
Application
Immunoblotting (1:1000)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Highly Toxic (H)
Physical form
In Tris-Citrate/phosphate, pH 7.0-8.0.
Reconstitution
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Analysis Note
Positive Control
Jurkat or NIH3T3 cells
Jurkat or NIH3T3 cells
Other Notes
Antibody should be titrated for optimal results in individual systems.
Mertens, A.E., et al. 2003. FEBS Lett.546, 11.
Qi, H., et al. 2001. Cell Growth Differ.12, 603.
Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.
Qi, H., et al. 2001. Cell Growth Differ.12, 603.
Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Regulatory Information
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Elena A Afanasyeva et al.
Life science alliance, 4(5) (2021-03-05)
The migrational propensity of neuroblastoma is affected by cell identity, but the mechanisms behind the divergence remain unknown. Using RNAi and time-lapse imaging, we show that ADRN-type NB cells exhibit RAC1- and kalirin-dependent nucleokinetic (NUC) migration that relies on several
Axel Walch et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 21(5), 544-552 (2008-02-05)
Rho GTPases are a family of major regulators of E-cadherin-mediated cell adhesion that are implicated in the carcinogenic process by deregulated expression of the family members itself or of upstream modulators or downstream effectors. Combined investigation of the Rho GTPase
Ramesh Chandra et al.
Frontiers in molecular neuroscience, 6, 13-13 (2013-06-08)
Exposure to psychostimulants results in structural and synaptic plasticity in striatal medium spiny neurons (MSNs). These cellular adaptations arise from alterations in genes that are highly implicated in the rearrangement of the actin-cytoskeleton, such as T-lymphoma invasion and metastasis 1
Davide Gianni et al.
Molecular biology of the cell, 19(7), 2984-2994 (2008-05-09)
NADPH oxidase (Nox) family enzymes are one of the main sources of cellular reactive oxygen species (ROS), which have been shown to function as second messenger molecules. To date, seven members of this family have been reported, including Nox1-5 and
Ahmed T Kurdi et al.
Nature communications, 7, 13048-13048 (2016-10-12)
RORγt is a master transcription factor of Th17 cells and considered as a promising drug target for the treatment of autoimmune diseases. Here, we show the guanine nucleotide exchange factor, Tiam1, and its cognate Rho-family G protein, Rac1, regulate interleukin
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