SNAP id^® 2.0 Protein Detection System

A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy1,2. The normal cellular function of ERα is as a transcription factor that mediates a wide variety of physiological processes, many of which are dependent upon phosphorylation of the receptor at specific amino acid residues3,4. Indeed, ERα is known to be phosphorylated at a multitude of different sites, yet how these all correlate to disease remains unclear5. Here, we interrogated multiple sites of ERα for phosphorylation status by screening an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.

"Immunohistochemistry (IHC) is a biochemical method used for the detection, localization and identification of biomarkers in tissue samples. Although IHC is used worldwide and many automated systems exist for handling large numbers of slides, research labs that manage small to medium numbers of slides at a time typically use the manual method, which can be tedious and timeconsuming, and the antibody used cannot be recovered for future use. The new SNAP i.d.® 2.0 Protein Detection System for immunohistochemistry is a versatile, vacuum-driven system that can process from 1 to 24 slides with formalin-fixed paraffin embedded tissue (FFPE) samples as well as fresh frozen samples. The system can accommodate any typical manual protocol, and eliminates the need for a Pap pen."