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Merck
CN

SF002

ESGRO Complete Basal Medium, 100mL

The ESGRO Complete Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF & BMP.

Synonym(s):

Mouse ES Cell Media

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.81
eCl@ss:
32160801

Key Documents

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Product Name

ESGRO Complete Basal Medium, 100mL, The ESGRO Complete Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF & BMP.

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

Quality Level

300

Application

Routine washing of cells.

Suitable for serum free culture of ES cells when used with LIF and BMP.

Suitable for use as a base medium for differentiation upon addition of differentiation factors of choice.

Medium suitable for use on feeder independent cell lines.

General description

ESGRO Complete™ Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF and BMP, and during routine washing steps when passaging cells.

Physical form

Basal serum free medium formulation without BMP or LIF.

pH 7.1-7.3

Preparation Note

Store at -20°C. Refer to lot expiration date on label. After thawing, store at 4°C for one month. DO NOT REFREEZE.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
cOmplete is a trademark of Roche

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Qi Long Ying et al.
Cell, 115(3), 281-292 (2003-11-26)
The cytokine leukemia inhibitory factor (LIF) drives self-renewal of mouse embryonic stem (ES) cells by activating the transcription factor STAT3. In serum-free cultures, however, LIF is insufficient to block neural differentiation and maintain pluripotency. Here, we report that bone morphogenetic
A critical appraisal of factors affecting the accuracy of results obtained when using flow cytometry in stem cell investigations: where do you put your gates?
Hughes O. R. et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology null

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