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SCT270

Sigma-Aldrich

BioTracker Far Red Exosome Protein Labeling Kit

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Synonym(s):
exosome protein dye, exosome protein label, exosome protein probe, far red exosome protein dye, far red exosome protein label, far red exosome protein probe, fluorescent exosome protein label
UNSPSC Code:
41106514
NACRES:
NA.47

biological source

human

Quality Level

packaging

vial of 5 tests (reactions)

manufacturer/tradename

Millipore

technique(s)

cell culture | stem cell: suitable
flow cytometry: suitable

shipped in

2-8°C

storage temp.

2-8°C

General description

"The BioTracker exosome protein labeling probe series can be used for staining the proteins of purified exosomes, which in turn permits imaging of labeled exosomes taken up by cells.SCT268, SCT269, and SCT270 are optimized for the labeling of exosomal proteins. Three different fluorescence colors (green, red, far red) enable application in multiplexing experiments, such as observation of exosome localization in organelles.The exosome labeling probe products contain filtration tubes for the removal of dyes unreacted after fluorescence labeling, as well as an optimized protocol for labeling exosomes. Add 10 uL DMSO to tube of green, red, or far red exosome protein dye to create stock solution.SPECTRAL PROPERTIES:Ex 640 nm/Em 640-760 nm

Features and Benefits

This live cell probe can be used for staining of purified exosomal protein, which allows imaging in the far red fluorescence range of labeled exosomes taken up by cells.

Target description

Exosomes are a form of extracellular vesicle (EV), which may be involved in malignant progression and the metastasis of cancer. Intercellular communication via exosomes has therefore attracted significant interest in the cancer research and cell communication disciplines. To elucidate these interactions, labeling techniques based on fluorescent dyes have been optimized.

Storage and Stability

Store BioTracker Far Red Exosome Protein Labeling Kit at 2 - 8 °C, protected from light.After resuspending in DMSO, store stock solution at -20 °C, protected from light.

Other Notes

SCT270 can be used for staining of purified exosomal protein, which allows imaging of labeled exosomes taken up by cells.Labeling capacity:Protein amount : 1-10μg/ sample, Particle count : 10 to 100 x 10^8 /sample(ultracentrifugation-purified exosome preparation)

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 3

Regulatory Information

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