SCT205
BioTracker™ EP-HMRG Green Protease Live Cell Probe
Synonym(s):
EP protease probe, EP-HMRG live cell probe, EP-HMRG probe, protease live cell probe
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About This Item
NACRES:
NB.12
UNSPSC Code:
41106514
manufacturer/tradename
Millipore
technique(s)
cell based assay: suitable
cell culture | mammalian: suitable
flow cytometry: suitable
shipped in
dry ice
storage temp.
−20°C
Quality Level
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Features and Benefits
Live cell imaging probe that reacts with the proteases that digest the peptide sequence of EP (glutamic acid, proline) to fluoresce green by fluorescence microscopy or flow cytometry.
General description
BioTracker™ EP-HMRG is nearly nonfluorescent at pH 7 or higher. It fluoresces by the reaction with proteases that specifically digest the EP (glutamic acid, proline) peptide sequence. A green fluorescent product is membrane permeable and localizes to the lysosome.To use the probe, warm the vial to room temperature and add 30 μL of DMSO or PBS to one vial to make 1 mM solution.Dilute the stock solution with a neutral buffer or culture medium to 1-2 uM and use the diluted solution immediately.SPECTRAL PROPERTIES:Excitation: 500 nmEmission: 524 nmFor fluorescence microscopy, blue excitation filter cubes for FITC or GFP are suitable.™ EP-HMRG jest prawie niefluorescencyjny przy pH 7 lub wyższym. Fluoryzuje w reakcji z proteazami, które specyficznie trawią sekwencję peptydową EP (kwas glutaminowy, prolina). Zielony fluorescencyjny produkt jest przepuszczalny dla błony i lokalizuje się w lizosomie. Aby użyć sondy, ogrzać fiolkę do temperatury pokojowej i dodać 30 μL DMSO lub PBS do jednej fiolki, aby uzyskać roztwór 1 mM. Rozcieńczyć roztwór podstawowy neutralnym buforem lub pożywką hodowlaną do 1-2 uM i natychmiast użyć rozcieńczonego roztworu. WŁAŚCIWOŚCI SPECJALNE: Wzbudzenie: 500 nm Emisja: 524 nm Do mikroskopii fluorescencyjnej odpowiednie są niebieskie kostki filtrów wzbudzenia dla FITC lub GFP. ODNIESIENIA Onoyama H, Kamiya M, Kuriki Y, Komatsu T, Abe H, Tsuji Y, Yagi K, Yamagata Y, Aikou S, Nishida M, et al. 2016. Szybkie i czułe wykrywanie wczesnego raka płaskonabłonkowego przełyku za pomocą sondy fluorescencyjnej ukierunkowanej na dipeptydylopeptydazę IV. Scientific Reports. 6(1):26399.
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Physical form
Lyophilized. Orange solid.
Preparation Note
Store BioTracker™ EP-HMRG Live Cell Probe at -20°C, desiccated and protected from light. Centrifuge vial briefly to collect contents at bottom of vial before opening.NOTE: Storage of reconstituted probe is not recommended. For best results, discard unused probe solution and make fresh for each use.
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
新产品
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