Skip to Content
Merck
CN

SCT064

BioTracker NTP-Transporter Molecule

Quick and efficient transport of labeled NTPs into cells for live cell imaging of DNA replication, synthesis, etc. Labeling of DNA with fluorescent NTPs is one of the essential methods in cell cycle analysis.

Synonym(s):

Cell cycle detection, Live cell imaging probe, Live cell imaging probe, Cell cylce probe

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

NACRES:
NA.47
UNSPSC Code:
12352207

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

BioTracker NTP-Transporter Molecule, Quick and efficient transport of labeled NTPs into cells for live cell imaging of DNA replication, synthesis, etc. Labeling of DNA with fluorescent NTPs is one of the essential methods in cell cycle analysis.

assay

≥98% (H-NMR)
≥98% (HPLC)
≥98% (LC/MS)
≥98% (elemental analysis)

form

solid

technique(s)

cell based assay: suitable

detection method

fluorometric

storage temp.

−20°C

Related Categories

Analysis Note

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 3470.14 g/mol

Application

Live cell fluorescent imaging
Quick and efficient transport of labeled NTPs into cells for live cell imaging of DNA replication, synthesis, etc. Labeling of DNA with fluorescent NTPs is one of the essential methods in cell cycle analysis.
Research Category
Cell Imaging
Research Sub Category
Cell Cycle, DNA Replication & Repair

DNA Damage & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nucleoside triphosphates (NTPs) are important metabolites that are involved in numerous cellular processes. However, due to their anionic nature, NTPs do not readily permeate cell membranes. To overcome this barrier, synthetic analogues that are neutral and more readily transportable are commonly used in cell-based assays. However, these synthetic analogues have a very low efficiency of conversion to active metabolites. Hence there is a need to optimize transport of NTPs into the cells.

BioTracker<TMSYMBOL></TMSYMBOL> NTP-Transporter Molecule has a receptor site, for selectively binding to the NTPs, and a cell penetrating agent. The transporter can bind to NTPs and quickly move them into cells without any apparent damage to the cell membrane or decrease in cell viability. The transported NTP is rapidly liberated from the complex and available in the nuclei for incorporation into DNA. Labeled NTPs can be used for live cell imaging, cell proliferation assays, synthetic biology studies, synthesizing artificial DNA, tracking cell division, research on active antiviral and anticancer nucleotides, among other things.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Lyophilized

Preparation Note

Store BioTracker NTP-Transporter Molecule at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

新产品
This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Zbigniew Zawada et al.
Angewandte Chemie (International ed. in English), 57(31), 9891-9895 (2018-03-27)
Chemically modified nucleoside triphosphates (NTPs) are widely exploited as unnatural metabolites in chemical biology and medicinal chemistry. Because anionic NTPs do not permeate cell membranes, their corresponding neutral precursors are employed in cell-based assays. These precursors become active metabolites after

Articles

Cell Cycle Analysis Using NTP Transporter

Cell cycle regulates vital processes like DNA repair, cancer prevention. Four stages: G1, S, G2, M. NTPs don't permeate membranes.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service