Skip to Content

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

For important updates on recent policy changes, please click here for more details.

Merck
CN
All Photos(2)

Key Documents

View All Documentation

Safety Information

SCR722

Millipore

B18R-E3L RNA (human codon optimized for B18R and E3L)

To strongly suppress more interferon responses than single B18R to enable more expression of a Simplicon® RNA or regular mRNA(s).

Sign Into View Organizational & Contract Pricing

Select a Size

25 EA
CN¥577.48

CN¥577.48

Please contact Customer Service for Availability
Sign In for Best Price
All Photos(2)

Select a Size

Change View
25 EA
CN¥577.48

About This Item

UNSPSC Code:
12352207
eCl@ss:
32161000
NACRES:
NA.51

CN¥577.48

Please contact Customer Service for Availability
Sign In for Best Price

technique(s)

nucleic acid detection: suitable (RNA)

shipped in

ambient

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
HCVD4MAG67KHCVD3MAG67KHCVD2MAG67K
MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 1 - Cardiovascular Disease Multiplex Assay The analytes available for this multiplex kit are: BNP, NT-proBNP, CK-MB, CXCL6/GCP-2, CXCL16, Endocan-1 (ESM-1), FABP3, FABP4, LIGHT, Oncostatin (OSM), Placental Growth Factor (PlGF), Troponin I (TnI).

HCVD1MAG67K

MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 1 - Cardiovascular Disease Multiplex Assay

MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 4 - Cardiovascular Disease Multiplex Assay The analytes available for this multiplex kit are: sE-Selectin, Follistatin, Pecam-1, Pentraxin-3, Tissue Factor (TF), Thrombomodulin, Troponin T (TnT).

HCVD4MAG67K

MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 4 - Cardiovascular Disease Multiplex Assay

MILLIPLEX® Human Cardiovascular Disease (Acute Phase) Magnetic Bead Panel 3 - Cardiovascular Disease Multiplex Assay The analytes available for this multiplex kit are: Adipsin, AGP, α2-Macroglobulin, CRP, Fetuin A, Fibrinogen, L-Selectin, Serum Amyloid P, Haptoglobin, & Platelet Factor-4.

HCVD3MAG67K

MILLIPLEX® Human Cardiovascular Disease (Acute Phase) Magnetic Bead Panel 3 - Cardiovascular Disease Multiplex Assay

MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay The analytes available for this multiplex kit are: ADAMTS13, D-Dimer, GDF-15, Myoglobin, sICAM-1, MPO, P-selectin, Lipocalin-2/NGAL, sVCAM-1, SAA.

HCVD2MAG67K

MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay

assay range

accuracy: 100%
(CK-MB), accuracy: 102%
(Oncostatin (OSM)), accuracy: 96%
(FABP3), accuracy: 97%
(NT proBNP), standard curve range: 2.7-2,000 pg/mL
(Placental Growth Factor (PlGF)), standard curve range: 6.9-5,000 pg/mL
(CXCL6, Oncostatin (OSM)), standard curve range: 685.9-500,000 pg/mL
(FABP4), inter-assay cv: <20%
intra-assay cv: <10%, accuracy: 103%
(CXCL6/GCP-2), standard curve range: 137.2-100,000 pg/mL
(FABP3), standard curve range: 68.6-50,000 pg/mL
(BNP, CK-MB, Troponin I (TnI)), accuracy: 102%
(LIGHT), standard curve range: 34.3-25,000 pg/mL
(NT proBNP, CXCL16, Endocan-1 (ESM-1), LIGHT), accuracy: 96%
(Placental Growth Factor (PlGF))

assay range

accuracy: 103%
(Thrombomodulin), accuracy: 104%
(Pentraxin-3 (PTX3)), accuracy: 104%
(Tissue Factor (TF)), accuracy: 106%
(Pecam-1), accuracy: 109%
(sE-Selectin), standard curve range: 0.01-5 ng/mL
(Troponin T (TnT)), standard curve range: 0.04-30 ng/mL
(Follistatin (FST)), standard curve range: 0.14-100 ng/mL
(Pentraxin-3 (PTX3) & Tissue Factor (TF)), standard curve range: 0.27-200 ng/mL
(Pecam-1), standard curve range: 0.34-250 ng/mL
(Thrombomodulin), standard curve range: 2.7-2,000 ng/mL
(sE-Selectin), inter-assay cv: <20%
intra-assay cv: <10%

assay range

accuracy: 102%
(Haptoglobin), accuracy: 109%
(AGP), accuracy: 113%
(Platelet Factor-4), accuracy: 117%
(Adipsin), standard curve range: 0.004-15 ng/mL
(L-Selectin & SAP), standard curve range: 0.012-50 ng/mL
(CRP), standard curve range: 0.037-150 ng/mL
(PF4), standard curve range: 0.061-250 ng/mL
(HPTGN), standard curve range: 0.098-400 ng/mL
(AGP & Adipsin), standard curve range: 0.244-1,000 ng/mL
(Fetuin A & vWF), standard curve range: 0.488-2,000 ng/mL
(A2M)

assay range

accuracy: 88%
(Myoglobin), accuracy: 90%
(P-selectin), accuracy: 98%
(GDF-15), standard curve range: 0.0005-2.5 ng/mL
(GDF-15), standard curve range: 0.012-50 ng/mL
(Lipocalcin-2/NGAL), standard curve range: 0.024-100 ng/mL
(Myoglobin & MPO), standard curve range: 0.085-350 ng/mL
(sICAM-1), standard curve range: 0.122-500 ng/mL
(sVCAM-1), standard curve range: 0.244-1,000 ng/mL
(D-dimer, SAA & P-selectin), standard curve range: 1.221-5,000 ng/mL
(ADAMTS 13), inter-assay cv: <20%
intra-assay cv: <15%

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Simplicon<TMSYMBOL></TMSYMBOL> is a novel system to effect immediate high sustained protein expression of multiple genes into transfected cells without the risk of genome integration. The technology employs a single, synthetic, polycistronic, self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome1,2,3. The Simplicon<TMSYMBOL></TMSYMBOL> RNA contains only genes encoding the VEE RNA replication machinery while the structural proteins that are required to make an infectious particle have been removed and replaced with the transgenes of interest.

Introduction and replication of the Simplicon<TMSYMBOL></TMSYMBOL> RNA is expected to elicit a strong interferon response in transfected cells. To suppress the IFN responses, a Vaccinia virus protein4, B18R, is used for the original Simplicon<TMSYMBOL></TMSYMBOL> technology. Recently, we found that another Vaccinia virus protein4, E3L, also suppresses the IFN responses in Simplicon<TMSYMBOL></TMSYMBOL> RNA expression. B18R neutralizes type I interferons by direct binding, while E3L inhibits the cytoplasmic signaling pathways of IFN responses. Therefore, B18R and E3L are both employed in the Simplicon<TMSYMBOL></TMSYMBOL> Expression System and work collaboratively to suppress IFN responses. As a result, there is increased cell viability during RNA transfection and increased expression of the transgenes. The Simplicon<TMSYMBOL></TMSYMBOL> Expression System works in human cells and is not expected to work in mouse cells. This is because the B18R does not effectively neutralize mouse interferon (IFN)-β.

The B18R-E3L RNA (human codon optimized for B18R and E3L) is a synthetic polycistronic mRNA that is co-transfected with the Simplicon<TMSYMBOL></TMSYMBOL> RNA to suppress the IFN responses. B18R-E3L RNA can also be used to prepare B18R conditioned medium by transfection into HFFs (Cat. No. SCC058). B18R-E3L RNA is also available for co-transfection of any mRNAs to suppress the IFN responses and enable repeated transfection of mRNAs. Please refer to the User Guide for Simplicon<TMSYMBOL></TMSYMBOL> Expression System located on our website.

Application

B18R-E3L RNA (human codon optimized for B18R and E3L) is provided as a polycistronic mRNA with both the B18R and E3L genes to more strongly suppress the interferon (IFN) responses that degrades Simplicon RNA(s) or regular mRNA(s). B18R RNA (E3L) works at single co-transfection with Simplicon RNA or repeated co-transfection with regular mRNA(s). B18R RNA (E3L) can also be used for production of B18R-CM (B18R-conditioned medium).
•Co-transfection with Simplicon RNA
•Co-transfection with mRNA for repeated transfection
•Production of B18R conditioned medium

Components

B18R-E3L RNA (human codon optimized for B18R and E3L), human codon optimized: (Cat. # CS224503), One (1) vial containing 10 µL of RNA (1 µg/µL). Store at -80 °C.

Quality

•Discrete RNA band on RNA gel of appropriate size.
•40-60% GFP-positive human foreskin fibroblasts (HFFs) with FACS analysis.

Storage and Stability

B18R-E3L RNA (human codon optimized for B18R and E3L): (Cat. # CS224503) One (1) vial containing 10 µL of RNA (1 µg/µL). Store at -80 °C.

Legal Information

SIMPLICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品

    • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

    Select a Product Number

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service