SCR140
Animal Free Collagenase/Dispase Blend II
Animal Free Collagenase/Dispase Blend II is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.
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Synonym(s):
MMP, Matrix metalloprotease, Dispase, Neutral Protease
UNSPSC Code:
12352204
eCl@ss:
32160405
NACRES:
NA.78
Recommended Products
Quality Level
caseinase activity
≥2000 caseinase units/mg
clostripain activity
≤5.0 clostripain units/mg
collagenase activity
≥12,500 U/vial
trypsin activity
≤0.5 trypsin units/mg
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
General description
Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types I, II, III, and IV or chromatographically purified collagenase. Dispase is especially suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. EMD Millipore’s Animal Free Collagenase/Dispase Blend II is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired. The product is prepared and purified from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens, such as BSE,TSE and other mammalian viruses, must be prevented.
Product Source: Isolated and purified from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents.
This product is manufactured in an ISO9001:2008 certified enviroment.
This product is manufactured in an ISO9001:2008 certified enviroment.
Application
Animal Free Collagenase/Dispase Blend II is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Enzymes & Biochemistry
Enzymes & Biochemistry
Physical form
Product is diafiltered with water, filtered through a 0.22 micron membrane and lyophilized in vials.
Storage and Stability
Store at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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