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SCR023

Sigma-Aldrich

Endothelial Cell Characterization Kit

Endothelial cell characterization kits enable researchers to characterize cultured endothelial cells in both a constitutive & activated state using basic ICC techniques.

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UNSPSC Code:
12352207
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

100

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable
immunocytochemistry: suitable

input

sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type cardiac stem cell(s)

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wet ice

General description

Endothelial Cell Characterization Kit

Antibodies for the immunocytochemical characterization of endothelial cells.
Endothelial cells form the inner lining of blood vessels and serve as the cellular interface between the circulating blood and the vessel wall. Endothelial cells are involved in many aspects of vascular biology, including inflammation, angiogenesis, atherosclerosis, blood clotting, vasoconstriction and vasodilation. Chemicon′s endothelial cell characterization kit enables researchers to characterize cultured endothelial cells in both a constitutive and activated state using basic immunocytochemistry techniques.

Endothelial cells express the following markers constitutively. For optimal results, the following antibody dilutions are recommended for immunocytochemistry:

* Mouse anti-Endothelial Cell (CD146): 1/500 dilution based on 1 mg/mL, final 2 ng/mL

* Rabbit anti-VWF: 1/5000 dilution based on 1 mg/mL, final 0.2 ng/mL

* Mouse anti-CD31: 1/50 dilution based on 0.1 mg/mL, final 20 ng/mL

The following antibodies have been shown to stain cultured HUVEC after 4-6hr of TNF-alpha (100ng/ml) induction at the following dilutions:

* Mouse anti-ICAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/mL

* Mouse anti-VCAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/Ml

Application

Research Category
Stem Cell Research

Components

50 μg of Endothelial Cell (CD146) monoclonal (Part No. 2003789)

50 μg of CD31 monoclonal (Part No. 2003788)

10 μg of von Willibrand Factor (vWF) polyclonal (Part No. 2003787)

50 μg of VCAM-1 monoclonal (Part No.MAB2511-50UG)

50 μg of ICAM-1 monoclonal (Part No. 2003785)

100 μg of Mouse control IgG (Part No. PP54-100UG)

100 μg of Rabbit control IgG (Part No. PP64-100UG)

Storage and Stability

Antibodies to ICAM-1, VCAM-1, von Willibrand Factor (vWF), and Endothelial Cells (CD146) are to be stored at 2 - 8°C (SCR023 - Part 1). Antibodies to CD31, Mouse IgG, and Rabbit IgG are to be stored at -20°C (SCR023 - Part 2).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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