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SCM111

Human ES/iPS Neuronal Differentiation Medium

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About This Item

UNSPSC Code:
41106200
eCl@ss:
32160801
NACRES:
NA.32

Key Documents

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form

liquid

Quality Level

100

technique(s)

cell differentiation: suitable

General description

EMD Millipore′s Human ES/iPS Neuronal Differentiation Medium is a serum free/chemically defined optimized media for the rapid production of terminally differentiated neurons from human neural progenitor cells. The media relies on both small molecules neural inducers and supplements to generate a highly enriched population of terimally differentiated TUJ-1/MAP2ab positive end stage neurons based on establish protocols (1,2).

Preparation Note

1) Neuronal Differentiation Basal Medium (Part No. CS211007). One 100 mL bottle. 2) Neural Supplement 1 (50X) (Part No. CS210992). One 2.5 mL bottle. 3) Adenosine 3′, 5′-cyclic Monophosphate, N6, O2′-Dibutyryl-, Sodium Salt (Cat. No. 28745-25mg). One vial containing 25 mg of lyophilized powder. 4) Ascorbic Acid 2-Phosphate (Part No. 2004011). One 0.5 mL vial of 100 mM solution.
Store at -20C. Good for 3 months from date of receipt or until expiration date on bottle when reagents are handled and stored appropriately. Please note: The supplemented ES/iPS Neuronal Differentiation Medium should be stored at 2 to 8C for up to 1 week.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

Regulatory Information

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Related Content

Neural Induction Medium Efficiently Generates Multipotent Neural Progenitors from Human iPS Cells

Dual SMAD inhibition is a well-established method to derive neural progenitor cells from both human ES and iPS cells. This protocol uses two SMAD inhibitors, Noggin and SB431542, to drive the rapid differentiation of ES/iPS cells into a highly enriched population of NPCs. Noggin acts as a BMP inhibitor and SB431542 inhibits the Lefty/Activin/TGFβ pathways by blocking the phosphorylation of ALK4, ALK5, and ALK7 receptors. In an effort to make a more defined and optimized neuronal differentiation protocol, Li and colleagues modified the original protocol to establish a completely small molecule-based differentiation method, which relies on three small molecules to inhibit GSK-3β (CHIR99021), TGFβ (SB431542), and Notch (compound E) signaling pathways, along with human LIF3. This new small molecule-based neural differentiation protocol increased neural differentiation kinetics and allowed the derivation of truly multipotent neural stem cells that respond to regional patterning cues specifying forebrain, midbrain, and hindbrain neural and glial subtypes.

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