SCM082
ES2N Complete Medium Kit
The ES2N Complete medium is a defined serum-free formulation that has been optimized for the differentiation of mouse embryonic stem (ES) & induced pluripotent stem (iPS) cells into neurons.
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ES2N Complete Medium Kit, The ES2N Complete medium is a defined serum-free formulation that has been optimized for the differentiation of mouse embryonic stem (ES) & induced pluripotent stem (iPS) cells into neurons.
form
liquid
technique(s)
cell culture | stem cell: suitable
input
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)
shipped in
dry ice
Quality Level
Analysis Note
Application
Disclaimer
General description
Other Notes
Neuro27 Medium Supplement: (Cat. No. SCM013-S) One (1) 5 mL vial containing neural supplement (use as 50x).
Neuro2 Medium Supplement: (Cat. No. SCM012-S) One (1) 1.25 mL vial containing N2-like supplement (use as 200x).
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
Certificates of Analysis (COA)
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Related Content
The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.
Neuroscience: Your Neural Network for Antibodies, Proteins, Kits, and Assays
Millipore’s new STEMCCA lentivirus reprogramming kits make it easier than ever to generate induced pluripotent stem (iPS) cells. Unlike traditional iPS generation which requires simultaneous co-infection by four separate expression vectors, the STEMCCA kits use a single polycistronic lentiviral vector to improve efficiency and reduce the number of viral integrations. The STEMCCA vector is comprised of the transcription factors Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences 1,2. It is also available with flanking LoxP sites incorporated for Cre-mediated excision of the exogenous reprogramming transgenes. STEMCCA Vector Advantages: (1) Efficient: uses a single vector with four transcription factors rather than co-transducing four separate expression vectors (2) Minimizes viral integrations: single vector reduces the risks of insertional mutagenesis and viral reactivation and (3) Excisable: Cre/LoxP-regulated version enables removal of reprogramming transgenes.
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