SCM003
Neural Stem Cell Basal Medium
The Neural Stem Cell Basal Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth & in vitro differentiation of neural stem cells derived from rodents.
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Synonym(s):
NSC Culture Media
UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.71
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Quality Level
form
liquid
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
input
sample type neural stem cell(s)
shipped in
dry ice
General description
The Neural Stem Cell Basal Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of neural stem cells derived from rodents. When used in conjunction with bFGF or bFGF, EGF and heparin, the basal medium will allow for the proliferation of rat and mouse neural stem cells, respectively. Withdrawal of the growth factors from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of rodent neural stem cells.
Application
The Neural Stem Cell Basal Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth & in vitro differentiation of neural stem cells derived from rodents.
Quality
Each lot of medium is tested for the optimal cell growth, proliferation and differentiation of both rat and mouse neural stem cells. Sterility Testing: Negative; Osmolarity: 299; pH: 7.4
Storage and Stability
Store at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jase Gehring et al.
Nature biotechnology, 38(1), 35-38 (2019-12-25)
We describe a universal sample multiplexing method for single-cell RNA sequencing in which fixed cells are chemically labeled by attaching identifying DNA oligonucleotides to cellular proteins. Analysis of a 96-plex perturbation experiment revealed changes in cell population structure and transcriptional
Protocols
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
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